Rabbit STAT2 Polyclonal Antibody | anti-STAT2 antibody

STAT2 Polyclonal Antibody

Cat. Num. AAA3013497

• Gene Names: STAT2; P113; IMD44; ISGF-3; STAT113
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: STAT2; Polyclonal Antibody; STAT2 Polyclonal Antibody; Homo sapiens interferon alpha induced transcriptional activator; interferon alpha induced transcriptional activator; ISGF 3; ISGF3; MGC59816; P113; signal transducer and activator of transcription 2 113kD; Signal transducer and activator of transcription 2; STAT113; Stat2; anti-STAT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: STAT2 polyclonal antibody detects endogenous levels of STAT2 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 851

• Applicable Applications for anti-STAT2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1,000; ICC: 1:50-1:200; IHC: 1:50-1:200
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Immunocytochemistry (ICC)

(ICC staining STAT2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining STAT2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-STAT2 antibody

Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3beta appears to be activated by both while Stat3alpha is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 4885615
• NCBI GeneID: 6773
• NCBI Accession #: NP_005410.1
• NCBI GenBank Nucleotide #: NP_005410.1
• UniProt Accession #: P52630; Q9WVL2
• Molecular Weight: 98kDa
• NCBI Official Full Name: signal transducer and activator of transcription 2 isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 2
• NCBI Official Symbol: STAT2
• NCBI Official Synonym Symbols: P113; IMD44; ISGF-3; STAT113
• NCBI Protein Information: signal transducer and activator of transcription 2
• UniProt Protein Name: Signal transducer and activator of transcription 2
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT2
• UniProt Entry Name: STAT2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

STAT2: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Binds to the IFN-stimulated response element (ISRE) and to the Gas element. This multiprotein transcription factor is termed ISGF3. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: identical protein binding; signal transducer activity; protein binding; DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; viral reproduction; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; JAK-STAT cascade; defense response to virus