Western Blot (WB)
(Western Blot analysis of PPP1CB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse skeletal muscle tissue lysateLane 2: Rat brain tissue lysateLane 3: A431 cell lysateLane 4: SH-SY-5Y cell lysateLane 5: SiHa cell lysateLane 6: 293 cell lysateLane 7: Hela cell lysateLane 8: PC-3M cell lysateLane 9: HepG2 cell lysateLane 10: A549 cell lysate)
Rabbit PPP1CB Polyclonal Antibody | anti-PPP1CB antibody
PPP1CB Polyclonal Antibody
IP: 1:50
IHC: 1:50-1:200
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of PPP1CB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse skeletal muscle tissue lysateLane 2: Rat brain tissue lysateLane 3: A431 cell lysateLane 4: SH-SY-5Y cell lysateLane 5: SiHa cell lysateLane 6: 293 cell lysateLane 7: Hela cell lysateLane 8: PC-3M cell lysateLane 9: HepG2 cell lysateLane 10: A549 cell lysate)
Western Blot (WB)
(Western Blot analysis of PPP1CB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse skeletal muscle tissue lysateLane 2: Rat brain tissue lysateLane 3: A431 cell lysateLane 4: SH-SY-5Y cell lysateLane 5: SiHa cell lysateLane 6: 293 cell lysateLane 7: Hela cell lysateLane 8: PC-3M cell lysateLane 9: HepG2 cell lysateLane 10: A549 cell lysate)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PPP1CB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ET7109-40 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PPP1CB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ET7109-40 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
PPP1CB: is a catalytic subunit of protein phosphatase 1 (PP1), a ubiquitous cytosolic serine-threonine phosphatase. Composed a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer's disease. Down-regulated in lung squamous cell carcinoma.
Protein type: Nucleolus; Motility/polarity/chemotaxis; EC 3.1.3.16; EC 3.1.3.53; Protein phosphatase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 2p23
Cellular Component: nucleoplasm; focal adhesion; glycogen granule; nucleolus; protein phosphatase type 1 complex; nucleus
Molecular Function: protein binding; myosin-light-chain-phosphatase activity; metal ion binding; phosphoric monoester hydrolase activity; myosin phosphatase activity; protein kinase binding
Biological Process: regulation of cell adhesion; glycogen metabolic process; regulation of glycogen biosynthetic process; protein amino acid dephosphorylation; regulation of circadian rhythm; entrainment of circadian clock by photoperiod; regulation of glycogen catabolic process; cell division; transforming growth factor beta receptor signaling pathway; triacylglycerol catabolic process; circadian regulation of gene expression; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of transforming growth factor beta receptor signaling pathway
Research Articles on PPP1CB
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Product Notes
The PPP1CB ppp1cb (Catalog #AAA3013286) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP1CB Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1CB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). WB: 1:1,000-1:10,000 IP: 1:50 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PPP1CB ppp1cb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP1CB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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