Rabbit LDHA Polyclonal Antibody | anti-LDHA antibody

LDHA Polyclonal Antibody

Cat. Num. AAA3012976

• Gene Names: LDHA; LDHM; GSD11; PIG19; HEL-S-133P
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide affinity purified
• Synonyms: LDHA; Polyclonal Antibody; LDHA Polyclonal Antibody; LDH-A; LDH-M; Cell proliferation-inducing gene 19 protein; GSD11; L lactate dehydrogenase A chain; L-lactate dehydrogenase A chain; l7R2; Lactate dehydrogenase 1; A chain; Lactate dehydrogenase A; Lactate dehydrogenase A4; Lactate dehydrogenase M; LDH A; LDH M; LDH muscle subunit; M LDH; LDH1; ldha; LDHM; OTTMUSP00000017774; PIG19; Proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59; anti-LDHA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LDHA polyclonal antibody detects endogenous levels of LDHA protein.
• Purity/Purification: Peptide affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 274

• Applicable Applications for anti-LDHA antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500; IHC: 1:200; FC/FACS: 1:100-1:200
• Immunogen: peptide
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of LDHA on different cell lysates using anti-LDHA antibody at 1/500 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: A431 Lane 4: 293 Lane 5: Jurkat Lane 6: MCF-7 Lane 7: Human liver Lane 8: Human kidney Lane 9: Human brain Lane 10: Human thymus)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-LDHA antibody. Counter stained with hematoxylin.)

Related Product Information for anti-LDHA antibody

Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor.

NCBI and Uniprot Product Information

• NCBI GI #: 207028494
• NCBI GeneID: 3939
• NCBI Accession #: NP_001128711.1
• NCBI GenBank Nucleotide #: NP_001128711.1
• UniProt Accession #: P00338
• Molecular Weight: 37kDa
• NCBI Official Full Name: L-lactate dehydrogenase A chain isoform 2
• NCBI Official Synonym Full Names: lactate dehydrogenase A
• NCBI Official Symbol: LDHA
• NCBI Official Synonym Symbols: LDHM; GSD11; PIG19; HEL-S-133P
• NCBI Protein Information: L-lactate dehydrogenase A chain
• UniProt Protein Name: L-lactate dehydrogenase A chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: LDHA
• UniProt Synonym Gene Names: LDH-A; LDH-M
• UniProt Entry Name: LDHA_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]