Western Blot (WB)
(Western Blot (WB) analysis of SRP19 polyclonal antibody)
Rabbit SRP19 Polyclonal Antibody | anti-SRP19 antibody
SRP19 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SRP19; Polyclonal Antibody; SRP19 Polyclonal Antibody; Signal recognition particle 19 kDa protein; anti-SRP19 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SRP19 polyclonal antibody detects endogenous levels of SRP19 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
110
Applicable Applications for anti-SRP19 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:10-1:100
IHC: 1:50-1:200
IF: 1:10-1:100
Immunogen
Recombinant full length Human SRP19.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of SRP19 polyclonal antibody)
Western Blot (WB)
(Western Blot (WB) analysis of SRP19 polyclonal antibody)
Related Product Information for anti-SRP19 antibody
Signal recognition particle (SRP) is a ribonucleoprotein composed of an Alu domain and an S domain that contains six proteins. The S domain contains unique sequence SRP RNA and four SRP proteins: SRP19, SRP54, SRP68 and SRP72. The Alu domain contains two SRP proteins, SRP9 and SRP14. SRP interacts with ribosomes to bring translating membrane and secreted proteins to the endoplasmic reticulum (ER) for proper processing. SRP9 and SRP14 form a heterodimer before binding to SRP RNA, and SRP19 functions in the assembly of SRP and binds to free SRP RNA. This event is a prerequisite for the subsequent binding of SRP54 to helix 8 of SRP RNA in eukaryotes and involves an SRP19-induced conformational change in the RNA.SRP54 interacts with both the nascent signal peptide and SRP RNA. SRP68 binding to SRP RNA enhances SRP72 binding. SRP19, SRP68 and SRP72 are localized in the nucleolus and cytoplasm, whereas SRP54 is only localized in the cytoplasm. SRP68 also accumulates in the ER. Thus, the nucleolus is the site of assembly and/or interaction between the family of ribonucleoproteins involved in protein synthesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 16kDa
NCBI Official Full Name
signal recognition particle 19 kDa protein isoform 2
NCBI Official Synonym Full Names
signal recognition particle 19
NCBI Official Symbol
SRP19
NCBI Protein Information
signal recognition particle 19 kDa protein
UniProt Protein Name
Signal recognition particle 19 kDa protein
Protein Family
UniProt Gene Name
SRP19
UniProt Synonym Gene Names
SRP19
UniProt Entry Name
SRP19_HUMAN
Uniprot Description
SRP19: Signal-recognition-particle assembly, binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. Belongs to the SRP19 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding
Chromosomal Location of Human Ortholog: 5q21-q22
Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleolus; signal recognition particle, endoplasmic reticulum targeting; cytosol; nucleus
Molecular Function: 7S RNA binding
Biological Process: response to drug; SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; gene expression; cotranslational protein targeting to membrane
Research Articles on SRP19
Similar Products
Product Notes
The SRP19 srp19 (Catalog #AAA3011715) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRP19 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRP19 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:10-1:100. Researchers should empirically determine the suitability of the SRP19 srp19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRP19, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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