Rabbit SEPX1 Polyclonal Antibody | anti-SEPX1 antibody

SEPX1 Polyclonal Antibody

Cat. Num. AAA3011705

• Gene Names: MSRB1; SELR; SELX; SepR; SEPX1; HSPC270
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SEPX1; Polyclonal Antibody; SEPX1 Polyclonal Antibody; Methionine-R-sulfoxide reductase B1; MsrB1; Selenoprotein X; SelX; MSRB1; HSPC270; anti-SEPX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SEPX1 polyclonal antibody detects endogenous levels of SEPX1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 116

• Applicable Applications for anti-SEPX1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human SEPX1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using MSRB1 Antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using SEPX1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-SEPX1 antibody

Selenium is an essential trace element that is incorporated as selenocysteine into the primary structure of selenoproteins. Nutritional deficiency of selenium decreases selenoprotein concentrations and leads to pathologic conditions. Most of the known selenoproteins are members of the glutathione peroxidase or iodothyronine deiodinase families. Selenoprotein P (SEPP1) is a major selenoprotein that is not a member of those families. It is an extracellular glycoprotein that is present in several isoforms and is the only selenoprotein known to contain multiple selenocysteine residues. SECIS elements form stem-loop structures in the 3' untranslated regions (UTR) of eukaryotic mRNAs that encode selenoproteins. The Selenoprotein W SECIS elements contain an additional highly conserved base-paired stem that may prevent inappropriate selenocysteine incorporation at the UGA stop codons. Selenoprotein R, also designated methionine-R-sulfoxide reductase or Selenoprotein X1, is a zinc binding protein. The gene encoding for Selenoprotein R contains seleno-cysteine, which is encoded by the usual stop codon TGA at the active site. Selenoprotein R may play a role in protection against oxidative stress.

NCBI and Uniprot Product Information

• NCBI GI #: 182676387
• NCBI GeneID: 51734
• UniProt Accession #: Q9NZV6
• Molecular Weight: ~ 13kDa
• NCBI Official Full Name: Methionine-R-sulfoxide reductase B1
• NCBI Official Synonym Full Names: methionine sulfoxide reductase B1
• NCBI Official Symbol: MSRB1
• NCBI Official Synonym Symbols: SELR; SELX; SepR; SEPX1; HSPC270
• NCBI Protein Information: methionine-R-sulfoxide reductase B1
• UniProt Protein Name: Methionine-R-sulfoxide reductase B1
• UniProt Gene Name: MSRB1
• UniProt Synonym Gene Names: SEPX1; MsrB1; SelX
• UniProt Entry Name: MSRB1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the methionine-R-sulfoxide reductase B (MsrB) family. Members of this family function as repair enzymes that protect proteins from oxidative stress by catalyzing the reduction of methionine-R-sulfoxides to methionines. This protein is highly expressed in liver and kidney, and is localized to the nucleus and cytosol. It is the only member of the MsrB family that is a selenoprotein, containing a selenocysteine (Sec) residue at its active site. It also has the highest methionine-R-sulfoxide reductase activity compared to other members containing cysteine in place of Sec. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A pseudogene of this locus has been identified on chromosome 19. [provided by RefSeq, Aug 2017]

Uniprot Description

SEPX1: a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the methionine sulfoxide reductase (Msr) protein family which includes repair enzymes that reduce oxidized methionine residues in proteins. The protein encoded by this gene is expressed in a variety of adult and fetal tissues and localizes to the cell nucleus and cytosol. [provided by RefSeq, Mar 2012]

Protein type: Oxidoreductase; EC 1.8.4.-

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; nucleus; actin cytoskeleton

Molecular Function: zinc ion binding; peptide-methionine (R)-S-oxide reductase activity; actin binding

Biological Process: protein repair; actin filament polymerization; innate immune response; response to oxidative stress