Rabbit RPL4 Polyclonal Antibody | anti-RPL4 antibody

RPL4 Polyclonal Antibody

Cat. Num. AAA3011469

• Gene Names: RPL4; L4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RPL4; Polyclonal Antibody; RPL4 Polyclonal Antibody; 60S ribosomal protein L4; 60S ribosomal protein L1; RPL1; anti-RPL4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RPL4 polyclonal antibody detects endogenous levels of RPL4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 427

• Applicable Applications for anti-RPL4 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human RPL4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RPL4 polyclonal antibody)

Related Product Information for anti-RPL4 antibody

Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L4, also known as RPL4 or RPL1, is a 427 amino acid cytoplasmic protein that is part of the large 60S ribosomal subunit complex. A member of the L4E family of ribosomal proteins, Ribosomal Protein L4 is thought to interact with the multifunctional nucleolar protein RNA helicase II (DDX21) and, through this interaction, may mediate the involvement of DDX21 in rRNA processing. Like most other ribosomal proteins, Ribosomal Protein L4 exists as multiple processed pseudogenes that are scattered throughout the genome.

NCBI and Uniprot Product Information

• NCBI GI #: 16579885
• NCBI GeneID: 6124
• NCBI Accession #: NP_000959.2
• NCBI GenBank Nucleotide #: NP_000959.2
• UniProt Accession #: P36578
• Molecular Weight: ~ 48kDa
• NCBI Official Full Name: 60S ribosomal protein L4
• NCBI Official Synonym Full Names: ribosomal protein L4
• NCBI Official Symbol: RPL4
• NCBI Official Synonym Symbols: L4
• NCBI Protein Information: 60S ribosomal protein L4
• UniProt Protein Name: 60S ribosomal protein L4
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL4
• UniProt Synonym Gene Names: RPL1
• UniProt Entry Name: RL4_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL4: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: focal adhesion; membrane; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; translational termination; viral infectious cycle