Rabbit Ataxin-1 Polyclonal Antibody | anti-ATXN1 antibody

Ataxin-1 Polyclonal Antibody

Cat. Num. AAA3011403

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Ataxin-1; Polyclonal Antibody; Ataxin-1 Polyclonal Antibody; alternative ataxin1; ATX1; ATX1_HUMAN; Atxn1; D6S504E; SCA1; Spinocerebellar ataxia type 1 protein; anti-ATXN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ataxin-1 polyclonal antibody detects endogenous levels of Ataxin-1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 815

• Applicable Applications for anti-ATXN1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human Ataxin-1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Ataxin-1 polyclonal antibody)

Related Product Information for anti-ATXN1 antibody

Ataxin-1, also designated spinocerebellar ataxia type 1 protein (Sca-1), is differentially expressed and localizes to both the cytoplasm and the nucleus. Mutations in Ataxin-1 are associated with the onset of the autosomal dominant neurodegenerative disorder spinocerebellar ataxia type 1 (SCA-1), which is characterized by progressive neuronal loss in the cerebellum, muscle wasting and ataxia. In Purkinje cells, where SCA-1 is predominantly observed, Ataxin-1 has been shown to directly associate with the Purkinje-enriched leucine-rich acidic nuclear protein (LANP) and the nuclear matrix-associated protein promyelocytic leukemia protein PML. In SCA-1, Ataxin-1 is mutated to encode a polyglutamine protein that forms nuclear aggregates, which interact significantly more strongly with LANP and contribute to the pathogenesis of SCA-1.

NCBI and Uniprot Product Information

• NCBI GI #: 51479158
• NCBI Accession #: NP_000323.2
• NCBI GenBank Nucleotide #: NP_000323.2
• UniProt Accession #: P54253
• Molecular Weight: ~ 84kDa
• NCBI Official Full Name: ataxin-1 ATXN1
• UniProt Protein Name: Ataxin-1
• Protein Family: Ataxin
• UniProt Gene Name: ATXN1
• UniProt Synonym Gene Names: ATX1; SCA1
• UniProt Entry Name: ATX1_HUMAN

Uniprot Description

ataxin-1: spinocerebellar ataxia type 1 (SCA1) protein. Expansion of CAG repeats in the SCA1 gene results in an abnormally long polyglutamine tract in the protein, causing polyglutamine-induced neurodegeneration. 14-3-3 and Akt kinase stabilize both wild-type and mutant forms of ataxin-1. The expansion of the polyglutamine tract may alter this function.

Protein type: RNA-binding; Nuclear export

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: nucleoplasm; nuclear RNA export factor complex; nuclear matrix; intracellular membrane-bound organelle; cytoplasm; nucleus; nuclear inclusion body

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; DNA binding; protein self-association; poly(U) binding; chromatin binding; poly(rG) binding

Biological Process: RNA processing; transcription, DNA-dependent; adult locomotory behavior; nuclear export; negative regulation of insulin-like growth factor receptor signaling pathway; negative regulation of phosphorylation; visual learning; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; regulation of excitatory postsynaptic membrane potential; negative regulation of transcription, DNA-dependent; alveolus development

Disease: Spinocerebellar Ataxia 1