Rabbit DDX20 Polyclonal Antibody | anti-DDX20 antibody

DDX20 Polyclonal Antibody

Cat. Num. AAA3011355

• Gene Names: DDX20; DP103; GEMIN3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DDX20; Polyclonal Antibody; DDX20 Polyclonal Antibody; Component of gems 3; DDX 20; ddx20; DDX20_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 20; DEAD box protein 20; DEAD box protein DP 103; DEAD box protein DP103; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20; DEAD/H BOX 20; DKFZP434H052; DP 103; DP103; Gemin-3; Gemin3; Probable ATP dependent RNA helicase DDX20; Probable ATP-dependent RNA helicase ddx20; SMN interacting protein; anti-DDX20 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DDX20 polyclonal antibody detects endogenous levels of DDX20 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 824

• Applicable Applications for anti-DDX20 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:10-1:100
• Immunogen: Recombinant full length Human DDX20.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DDX20 polyclonal antibody)

Related Product Information for anti-DDX20 antibody

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of motor neurons in the spinal cord. SMA is caused by deletion or loss-of-function mutations in the SMN (survival of motor neuron) gene. Gemin3, also known as DP103, DDX20, DEAD-box protein DP130 and DEAD/H box 20, is a protein product of human chromosome 1p13.2. It associates directly with SMN and is a part of the SMN complex containing Gemin2, Gemin4, Gemin5 and Gemin6, as well as several spliceosomal snRNP proteins. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing of the nucleus. It is found in both the cytoplasm and the nucleus. The nuclear form is concentrated in subnuclear bodies called gems (for Gemini of the coiled bodies). Gemin3 also interacts with SmB, SmD2 and SmD3. It contains the conserved motif Asp-Glu-Ala-Asp (DEAD) characteristic of DEAD-box proteins. Gemin3 is a putative RNA helicase and shows ATPase activity. It is expressed in B and T cell neuroblastoma-derived cell lines, malignant melanoma tumor, normal testis and is expressed in low levels in colon, skeletal muscle, liver, kidney and lung.

NCBI and Uniprot Product Information

• NCBI GI #: 256223453
• NCBI GeneID: 11218
• NCBI Accession #: NP_009135.4
• NCBI GenBank Nucleotide #: NP_009135.4
• UniProt Accession #: Q9UHI6
• Molecular Weight: ~ 110kDa
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX20
• NCBI Official Synonym Full Names: DEAD-box helicase 20
• NCBI Official Symbol: DDX20
• NCBI Official Synonym Symbols: DP103; GEMIN3
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX20
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX20
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX20
• UniProt Synonym Gene Names: DP103; GEMIN3
• UniProt Entry Name: DDX20_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq, Jul 2008]

Uniprot Description

GEMIN3: an ubiquitous DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. Interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.

Protein type: EC 3.6.4.13; Helicase; RNA splicing; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p21.1-p13.2

Cellular Component: nucleoplasm; cytoskeleton; transcriptional repressor complex; membrane; cytoplasm; SMN complex; nucleus; cytosol

Molecular Function: protein domain specific binding; protein binding; DNA binding; ATP binding; ATP-dependent RNA helicase activity

Biological Process: RNA processing; assembly of spliceosomal tri-snRNP; oogenesis; spliceosomal snRNP biogenesis; positive regulation of apoptosis; regulation of steroid biosynthetic process; gene expression; negative regulation of transcription from RNA polymerase II promoter