Rabbit anti-Human DUT Polyclonal Antibody | anti-DUT antibody

DUT Polyclonal Antibody

Cat. Num. AAA3011266

• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DUT; Polyclonal Antibody; DUT Polyclonal Antibody; Deoxyuridine triphosphatase; dut; DUT_HUMAN; dUTP nucleotidohydrolase; dUTP pyrophosphatase; dUTPase; anti-DUT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DUT polyclonal antibody detects endogenous levels of DUT protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 252

• Applicable Applications for anti-DUT antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human DUT.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DUT polyclonal antibody)

Testing Data

Related Product Information for anti-DUT antibody

dUTPase (deoxyuridine 5-triphosphate nucleotidohydrolase), also known as DUT or dUTP pyrophosphatase, is a preventive DNA repair enzyme that functions in nucleotide metabolism. dUTPase is expressed in a variety of tissues and, depending on the isoform (DUT-N or DUT-M), localizes to the nucleus or the mitochondrion. The nuclear isoform, DUT-N, is the most abundant of the two isoforms. dUTPase, in the presence of magnesium ions, is responsible for hydrolyzing dUTP to dUMP and diphosphate. This reaction is important for keeping the intracellular dUTP concentration low so that uracil does not become incorporated into DNA. Extensive incorporation of uracil into DNA can ultimately lead to cell death. This suggests that dUTPase is essential for cell viability, further implying that dUTPase is a potential target for anticancer therapy. In addition, dUMP, the product of the hydrolysis reaction, is a precursor of thymidine nucleotides which are essential for DNA replication.

NCBI and Uniprot Product Information

• NCBI GI #: 1812588782
• UniProt Accession #: P33316
• Molecular Weight: ~ 24kDa
• NCBI Official Full Name: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
• UniProt Protein Name: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
• Protein Family: Deoxyuridine 5'-triphosphate nucleotidohydrolase
• UniProt Gene Name: DUT
• UniProt Synonym Gene Names: dUTPase
• UniProt Entry Name: DUT_HUMAN

Uniprot Description

dUTPase: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Belongs to the dUTPase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Motility/polarity/chemotaxis; Mitochondrial; DNA replication; Nuclear receptor co-regulator; Nucleotide Metabolism - pyrimidine; EC 3.6.1.23

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function: protein binding; magnesium ion binding; dUTP diphosphatase activity

Biological Process: dUTP catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; DNA replication; dUMP biosynthetic process