Western Blot (WB)
(Western Blot analysis of NOLC1 antibody.)
Rabbit anti-Human NOLC1 Polyclonal Antibody | anti-NOLC1 antibody
NOLC1 Polyclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
NOLC1; Polyclonal Antibody; NOLC1 Polyclonal Antibody; P130; NOPP130; NOPP140; NS5ATP13; anti-NOLC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
NOLC1 polyclonal antibody detects endogenous levels of NOLC1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
699
Applicable Applications for anti-NOLC1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human NOLC1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of NOLC1 antibody.)
Western Blot (WB)
(Western Blot analysis of NOLC1 antibody.)
Related Product Information for anti-NOLC1 antibody
Nopp140, previously named p130, is a nucleolar phosphoprotein that has been shown to exist in multiple forms with different sizes. Nopp140 functions both as a chaperone for import and/or export from the nucleolus and as a transcription factor. Nopp140 was originally identified from rat liver as an NLS (nuclear localization signal)-binding protein, and has been further characterized as an RNAP (RNA Polymerase)-interacting protein. Nopp140 also associates with the general transcription factor TFIIB, and the protein kinase casein kinase II (CKII). CKII heavily phosphorylates Nopp140 to mediate binding of Nopp140 to NLS. Nopp 140 colocalizes with another nucleolar protein, NAP57, in the nucleolus and coiled bodies, and is thought to be involved in activities carried out within the nucleolus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 120kDa
NCBI Official Full Name
nucleolar and coiled-body phosphoprotein 1 isoform 2
UniProt Protein Name
Nucleolar and coiled-body phosphoprotein 1
Protein Family
UniProt Gene Name
NOLC1
UniProt Synonym Gene Names
KIAA0035; NS5ATP13; Nopp140
UniProt Entry Name
NOLC1_HUMAN
Uniprot Description
NOLC1: Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Nucleolus; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 10q24.32
Cellular Component: nucleoplasm; Cajal body; cytoplasm; nucleolus
Molecular Function: protein binding; GTP binding; ATP binding
Biological Process: mitosis; cell cycle; rRNA processing; nucleolus organization and biogenesis
Similar Products
Product Notes
The NOLC1 nolc1 (Catalog #AAA3008672) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOLC1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOLC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NOLC1 nolc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOLC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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