Rabbit IGF2BP1 Polyclonal Antibody | anti-IGF2BP1 antibody

IGF2BP1 Polyclonal Antibody

Cat. Num. AAA3008231

• Gene Names: IGF2BP1; IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IGF2BP1; Polyclonal Antibody; IGF2BP1 Polyclonal Antibody; Insulin-like growth factor-II mRNA-binding proteins (IMPs) belong to a family of zipcode-binding proteins. Three members of this family; IMP1; IMP2; and IMP3; have been identified. They contain two RNA recognition motifs; four K homology domains; and were found to function in mRNA localization; turnover; and translation control. Research studies have implicated these proteins in a variety of physiological and pathological processes; such as growth and development; testicular neoplasia; and melan; anti-IGF2BP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IGF2BP1 polyclonal antibody detects endogenous levels of IGF2BP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 577

• Applicable Applications for anti-IGF2BP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:100; IF: 1:50-1:200
• Immunogen: Recombinant full length Human IGF2BP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of IGF2BP1 polyclonal antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of IGF2BP1 polyclonal antibody in paraffin-embedded Human Esophageal Cancer tissue.)

Related Product Information for anti-IGF2BP1 antibody

IGF-II mRNA-binding proteins (IMP) bind RNA and influence RNA synthesis and metabolism. IMPs, IMP-1 (coding region determinant-binding protein/insulin-like growth factor II mRNA-binding protein, CRD-BP, VICKZ1), IMP-2 (IMP2, VICKZ2, p62) and IMP-3 (KOC1, VICKZ3), contain a unique combination of RNA recognition motifs and four hnRNP K homology domains. IMP-1 is abundant in embryonal tissues and in 81% of colon cancers, 58.5% of breast cancers and 73% of sarcomas. IMP-1 recognizes c-Myc, IGF-II and tau mRNAs, and H19 RNA and plays a major role in proliferation of K-562 cells by an IGF-II-dependent mechanism. IMP-2 binds the 5' UTR of IGF-II mRNA and influences tumor cell growth, in which IMP-2 is associated with apoptosis induced by tretinoin. IMP-3 knock down by RNA interference decreases levels of IGF-II protein without affecting IGF-II, c-Myc, or b Actin mRNA and H19 RNA levels. IMP-3 is a marker for carcinomas and high-grade dysplastic lesions of pancreatic ductal epithelium.

NCBI and Uniprot Product Information

• NCBI GI #: 296434536
• NCBI GeneID: 10642
• UniProt Accession #: Q9NZI8
• Molecular Weight: ~ 70kDa
• NCBI Official Full Name: Insulin-like growth factor 2 mRNA-binding protein 1
• NCBI Official Synonym Full Names: insulin like growth factor 2 mRNA binding protein 1
• NCBI Official Symbol: IGF2BP1
• NCBI Official Synonym Symbols: IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
• NCBI Protein Information: insulin-like growth factor 2 mRNA-binding protein 1
• UniProt Protein Name: Insulin-like growth factor 2 mRNA-binding protein 1
• Protein Family: Insulin-like growth factor 2 mRNA-binding protein
• UniProt Gene Name: IGF2BP1
• UniProt Synonym Gene Names: CRDBP; VICKZ1; ZBP1; IGF2 mRNA-binding protein 1; IMP-1; IMP1; CRD-BP; ZBP-1
• UniProt Entry Name: IF2B1_HUMAN

NCBI Description

This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

IGF2BP1: a conserved trans-acting factor that can control translation of specific mRNAs in vivo. Contains four K homology (KH) domains and two RNA recognition motifs. It functions by binding to the 5' UTR of the target mRNAs and repressing translation. Target mRNAs include those encoding beta-actin and insulin-like growth factor 2 (IGF2). Tyrosine phosphorylation apparently abolishes translational repression of actin expression by decreasing its binding beta-actin mRNA. May have a role in progression of ovarian cancer. Contains nuclear export signals within the RNA-binding KH2 and KH4 domains. Aberrant expression may interfere with c-myc regulation.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: growth cone; perinuclear region of cytoplasm; stress granule; axon; lamellipodium; cytoplasm; dendritic spine; ribonucleoprotein complex; cytosol; nucleus; filopodium

Molecular Function: mRNA binding; protein binding; mRNA 3'-UTR binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: mRNA transport; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process