Rabbit anti-Human, Mouse ECI1 Polyclonal Antibody | anti-ECI1 antibody

ECI1 Polyclonal Antibody

Cat. Num. AAA3007785

• Gene Names: ECI1; DCI
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ECI1; Polyclonal Antibody; ECI1 Polyclonal Antibody; ACBD2; acyl Coenzyme A binding domain containing 2; D3 D2 enoyl CoA isomerase; DBI related protein 1; Delta(3) delta(2) enoyl CoA isomerase; Diazepam-binding inhibitor-related protein 1; Dodecenoyl CoA isomerase; DRS 1; DRS1; HCA88; Hepatocellular carcinoma associated antigen 88; Peroxisomal 3; 2-trans-enoyl-CoA isomerase; Peroxisomal D3 D2 enoyl CoA isomerase; Renal carcinoma antigen NY REN 1; anti-ECI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ECI1 polyclonal antibody detects endogenous levels of ECI1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 302

• Applicable Applications for anti-ECI1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human ECI1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ECI1 polyclonal antibody)

Immunofluorescence (IF)

(Immunofluorescence analysis of Hela cells, using ECI1 polyclonal antibody)

Related Product Information for anti-ECI1 antibody

Protein names: Enoyl-CoA delta isomerase 1, mitochondrial; 3, 2-trans-enoyl-CoA isomerase; Delta(3), Delta(2)-enoyl-CoA isomerase; Short name=D3, D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase. Gene names: ECI1, DCI. Function: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Catalytic activity: (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA. Pathway: Lipid metabolism; fatty acid beta-oxidation. Subunit structure: Homotrimer. Subcellular location: Mitochondrion matrix.

NCBI and Uniprot Product Information

• NCBI GI #: 1169204
• NCBI GeneID: 1632
• UniProt Accession #: P42126
• Molecular Weight: ~ 34kDa
• NCBI Official Full Name: Enoyl-CoA delta isomerase 1, mitochondrial
• NCBI Official Synonym Full Names: enoyl-CoA delta isomerase 1
• NCBI Official Symbol: ECI1
• NCBI Official Synonym Symbols: DCI
• NCBI Protein Information: enoyl-CoA delta isomerase 1, mitochondrial
• UniProt Protein Name: Enoyl-CoA delta isomerase 1, mitochondrial
• Protein Family: Enoyl-CoA delta isomerase
• UniProt Gene Name: ECI1
• UniProt Synonym Gene Names: DCI; D3,D2-enoyl-CoA isomerase
• UniProt Entry Name: ECI1_HUMAN

NCBI Description

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]

Uniprot Description

DCI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Belongs to the enoyl-CoA hydratase/isomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 5.3.3.8; Isomerase; Mitochondrial; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: dodecenoyl-CoA delta-isomerase activity; intramolecular oxidoreductase activity

Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process