Rabbit UBE3B Polyclonal Antibody | anti-UBE3B antibody

UBE3B Polyclonal Antibody

Cat. Num. AAA3007583

• Gene Names: UBE3B; KOS; BPIDS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UBE3B; Polyclonal Antibody; UBE3B Polyclonal Antibody; Ubiquitin-protein ligase E3B; HECT-type ubiquitin transferase E3B; anti-UBE3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UBE3B polyclonal antibody detects endogenous levels of UBE3B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 244

• Applicable Applications for anti-UBE3B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human UBE3B.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of UBE3B polyclonal antibody at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug))

Related Product Information for anti-UBE3B antibody

Ubiquitin-protein ligase E3B, E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

NCBI and Uniprot Product Information

• NCBI GI #: 394025733
• NCBI GeneID: 89910
• NCBI Accession #: NP_001257378.1
• NCBI GenBank Nucleotide #: NP_001257378.1
• UniProt Accession #: Q7Z3V4
• Molecular Weight: ~ 123kDa
• NCBI Official Full Name: ubiquitin-protein ligase E3B isoform 3
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3B
• NCBI Official Symbol: UBE3B
• NCBI Official Synonym Symbols: KOS; BPIDS
• NCBI Protein Information: ubiquitin-protein ligase E3B
• UniProt Protein Name: Ubiquitin-protein ligase E3B
• Protein Family: Ubiquitin-protein ligase
• UniProt Gene Name: UBE3B
• UniProt Entry Name: UBE3B_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2012]