Rabbit anti-Human IKZF3 Polyclonal Antibody | anti-IKZF3 antibody

IKZF3 Polyclonal Antibody

Cat. Num. AAA3007455

• Gene Names: IKZF3; AIO; AIOLOS; ZNFN1A3
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: IKZF3; Polyclonal Antibody; IKZF3 Polyclonal Antibody; Zinc finger protein Aiolos; Ikaros family zinc finger protein 3; anti-IKZF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IKZF3 polyclonal antibody detects endogenous levels of IKZF3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 475

• Applicable Applications for anti-IKZF3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 363-407 of Human IKZF3
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of IKZF3 polyclonal antibody at 1:500 dilutionLane1:Jurkat whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug))

Related Product Information for anti-IKZF3 antibody

Aiolos is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family members. Multiple Aiolos isoforms are generated through alternative splicing of the portion of the transcript encoding the amino-terminal zinc fingers. Aiolos is expressed by lymphoid tissues, with highest expression levels seen in mature B and T cells. Ikaros family proteins control lymphocyte development by recruiting chromatin remodeling complexes to DNA. B cells from mice lacking Aiolos have a reduced threshold for activation, increased proliferation, and elevated levels of IgG and IgE. In addition, Aiolos null mice develop B cell lymphomas. In T cells, Aiolos contributes to Th17 cell differentiation by suppressing IL-2 expression. Aberrant expression of Aiolos in transformed epithelial cells promotes anchorage independence through downregulation of adhesion-related genes. Alterations in the Aiolos gene are observed in near haploid acute lymphoblastic leukemia, and the genetic locus containing Aiolos is linked to increased susceptibility to rheumatoid arthritis and systemic lupus erythematosus.

NCBI and Uniprot Product Information

• NCBI GI #: 383872639
• NCBI GeneID: 22806
• NCBI Accession #: NP_001244337.1
• NCBI GenBank Nucleotide #: NP_001244337.1
• UniProt Accession #: Q9UKT9
• Molecular Weight: ~ 50, 58, 65kDa
• NCBI Official Full Name: zinc finger protein Aiolos isoform 7
• NCBI Official Synonym Full Names: IKAROS family zinc finger 3
• NCBI Official Symbol: IKZF3
• NCBI Official Synonym Symbols: AIO; AIOLOS; ZNFN1A3
• NCBI Protein Information: zinc finger protein Aiolos
• UniProt Protein Name: Zinc finger protein Aiolos
• Protein Family: Zinc finger protein
• UniProt Gene Name: IKZF3
• UniProt Synonym Gene Names: ZNFN1A3
• UniProt Entry Name: IKZF3_HUMAN

NCBI Description

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants. [provided by RefSeq, Apr 2012]

Uniprot Description

Aiolos: a transcription factor of the ikaros C2H2-type zinc-finger protein family. Plays an important role in the regulation of lymphocyte differentiation. Deletions in Aiolos have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; sequence-specific DNA binding; metal ion binding; transcription factor activity

Biological Process: regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; B cell activation; regulation of lymphocyte differentiation; regulation of B cell differentiation; regulation of B cell proliferation; mesoderm development