Rabbit EPHB1/EPHB2/EPHB3 Polyclonal Antibody | anti-EPHB1 antibody

EPHB1/EPHB2/EPHB3 polyclonal antibody

Cat. Num. AAA3007351

• Gene Names: EPHB1; ELK; NET; Hek6; EPHT2
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: EPHB1/EPHB2/EPHB3; Polyclonal Antibody; EPHB1/EPHB2/EPHB3 polyclonal antibody; Ephrin type-B receptor 1; ELK; EPH tyrosine kinase 2; EPH-like kinase 6; EK6; hEK6; Neuronally-expressed EPH-related tyrosine kinase; NET; Tyrosine-protein kinase receptor EPH-2; EPHB1; EPHT2; HEK6; Ephrin type-B receptor 2; Developmentally-regulated Eph-related tyrosine kinase; ELK-related tyrosine kinase; EPH tyrosine kinase 3EPH-like kinase 5; EK5; hEK5; Renal carcinoma antigen NY-REN-47; Tyrosine-protein kinase TYRO5; Tyrosine-protein kinase receptor EPH-3; EphB2/CTF1; EphB2/CTF2; EPHB2; DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5; Ephrin type-B receptor 3; EPH-like tyrosine kinase 2; EPH-like kinase 2; Embryonic kinase 2; EK2; hEK2; Tyrosine-protein kinase TYRO6; EPHB3; ETK2; HEK2; TYRO6; anti-EPHB1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EPHB1/EPHB2/EPHB3 polyclonal antibody detects endogenous levels of EPHB1/EPHB2/EPHB3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
• Sequence Length: 984

• Applicable Applications for anti-EPHB1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to Human EPHB1/EPHB2/EPHB3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of EPHB1/EPHB2/EPHB3 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate (30ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:The Heart tissue lysate of Rat (30ug) Lane4:The Brain tissue lysate of Mouse (40ug))

Related Product Information for anti-EPHB1 antibody

The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain. Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer. Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered. The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells. Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF. Tyr324 and Tyr327 have been identified as major phosphorylation sites of ephrin B1 in vivo. EphB1 is a member of the Eph family of receptor tyrosine kinases that plays an important role in diverse biological processes including nervous system development, angiogenesis, and neural synapsis formation and maturation. Over- or underexpression of certain Eph receptors has been found in some cancer tissues. EphB1 has been shown to be involved in the tumorigenesis of colorectal cancer.The ephrin receptor B2 (EphB2) is an ephrin family receptor tyrosine kinase that plays an important role in regulating growth and development of multiple tissues and organs. The EphB2 transmembrane receptor protein contains a kinase domain, a PDZ motif, and a SAM domain within a conserved cytoplasmic domain. A ligand binding domain, a cysteine-rich domain, and fibronectin type III repeats comprise the conserved EphB2 extracellular domain. EphB2 binds with high affinity to ephrin B ligands, and to some ephrin A proteins, to initiate bidirectional signaling between neighboring cells. Upon binding, EphB2-Ephrin B2 dimers form a heterotetramer and position the receptor-ligand complex on the cell membrane to facilitate bidirectional signal transduction. In addition to associating with ephrin ligands, EphB2 also regulates a number of biological processes through interaction with focal adhesion kinase (FAK), NMDA receptor (NMDAR), the Rac1 guanine nucleotide exchange factor Tiam1, and p21-activated kinase (PAK1). While some studies support a role for EphB2 as a pro-oncogenic kinase, other research suggests that EphB2 acts as a tumor suppressor.

NCBI and Uniprot Product Information

• NCBI GI #: 4758284
• NCBI GeneID: 2047
• NCBI Accession #: NP_004432.1
• NCBI GenBank Nucleotide #: NP_004432.1
• UniProt Accession #: P54762; P29323; P54753
• Molecular Weight: ~ 130kDa
• NCBI Official Full Name: ephrin type-B receptor 1
• NCBI Official Synonym Full Names: EPH receptor B1
• NCBI Official Symbol: EPHB1
• NCBI Official Synonym Symbols: ELK; NET; Hek6; EPHT2
• NCBI Protein Information: ephrin type-B receptor 1
• UniProt Protein Name: Ephrin type-B receptor 1
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB1
• UniProt Synonym Gene Names: ELK; EPHT2; HEK6; NET; EK6; hEK6; NET
• UniProt Entry Name: EPHB1_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]

Uniprot Description

EphB1: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-B family: ephrin-B1, -B2 and -B3. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB1 interacts with GRB2, GRB10 and NCK through their respective SH2 domains. Four alternatively spliced isoforms are known.

Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 3q21-q23

Cellular Component: early endosome membrane; integral to plasma membrane; axon; dendrite; extracellular region; plasma membrane; cytosol; lipid raft

Molecular Function: protein binding; transmembrane-ephrin receptor activity; axon guidance receptor activity; ATP binding

Biological Process: positive regulation of synaptogenesis; axon guidance; camera-type eye morphogenesis; peptidyl-tyrosine phosphorylation; neurogenesis; establishment of cell polarity; protein amino acid autophosphorylation; ephrin receptor signaling pathway; central nervous system projection neuron axonogenesis; angiogenesis; optic nerve morphogenesis; detection of temperature stimulus involved in sensory perception of pain; regulation of JNK cascade; cell-substrate adhesion; retinal ganglion cell axon guidance