Rabbit BAX Polyclonal Antibody | anti-BAX antibody

BAX Polyclonal Antibody

Cat. Num. AAA3007219

• Gene Names: BAX; BCL2L4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BAX; Polyclonal Antibody; BAX Polyclonal Antibody; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BCL2L4; anti-BAX antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BAX polyclonal antibody detects endogenous levels of BAX protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 218

• Applicable Applications for anti-BAX antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human BAX.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BAX polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysateLane4:HELA whole cell lysate)

Related Product Information for anti-BAX antibody

The Bax protein belongs to a growing family of related Bcl-2 proteins which regulate apoptosis by controlling the permeability of the mitochondrial membrane to the apoptogenic protein cytochrome c. Cytochrome c, in turn, activates the proteolytic proteins known as caspases. The Bcl-2 protein family members play both pro-apoptotic and anti-apoptotic roles, and all possess at least one of four conserved motifs known as "Bcl-2 homology domains" (BH1 to BH4). These domains play key roles as binding sites which allow these proteins to form homodimers or heterodimers, thus regulating the apoptotic activity of these proteins. Bax is a cytosolic protein that plays a pro-apoptotic role by binding to the permeability transition pore complex (PTPC) and by binding Bcl-2, a protein which plays an anti-apoptotic role. The ratio between Bax/Bcl-2 heterodimers and Bax/Bax homodimers appears to be pivotal in determining the lifespan of a cell. Bax exhibits numerous splice variants, including alpha, beta, delta, gamma and kappa. Bax-beta is a 218 amino acid protein. Intron 5 of Bax-beta RNA consists of 630 bp and does not undergo splicing, which accounts for the apparent size increase.

NCBI and Uniprot Product Information

• NCBI GI #: 4757838
• NCBI GeneID: 581
• NCBI Accession #: NP_004315.1
• NCBI GenBank Nucleotide #: NP_004315.1
• UniProt Accession #: Q07815; Q07812
• Molecular Weight: ~ 18kDa
• NCBI Official Full Name: apoptosis regulator BAX isoform beta
• NCBI Official Synonym Full Names: BCL2 associated X, apoptosis regulator
• NCBI Official Symbol: BAX
• NCBI Official Synonym Symbols: BCL2L4
• NCBI Protein Information: apoptosis regulator BAX
• UniProt Protein Name: Apoptosis regulator BAX
• Protein Family: Bax inhibitor
• UniProt Gene Name: BAX
• UniProt Synonym Gene Names: BCL2L4; Bcl2-L-4
• UniProt Entry Name: BAX_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Dec 2019]

Uniprot Description

BAX: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37. Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Belongs to the Bcl-2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Apoptosis; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial permeability transition pore complex; pore complex; endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; mitochondrion; endoplasmic reticulum; nuclear envelope; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; channel activity; BH3 domain binding; lipid binding

Biological Process: hypothalamus development; viral reproduction; regulation of cell cycle; positive regulation of apoptosis; response to toxin; germ cell programmed cell death; myeloid cell homeostasis; B cell apoptosis; germ cell development; regulation of mammary gland epithelial cell proliferation; spermatid differentiation; regulation of mitochondrial membrane potential; development of secondary sexual characteristics; protein insertion into mitochondrial membrane during induction of apoptosis; establishment and/or maintenance of transmembrane electrochemical gradient; negative regulation of neuron apoptosis; negative regulation of protein binding; kidney development; release of cytochrome c from mitochondria; positive regulation of B cell apoptosis; regulation of protein homodimerization activity; vagina development; protein oligomerization; unfolded protein response, activation of signaling protein activity; fertilization; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; retina development in camera-type eye; negative regulation of fibroblast proliferation; reduction of endoplasmic reticulum calcium ion concentration; glycosphingolipid metabolic process; cerebral cortex development; mitochondrial fragmentation during apoptosis; regulation of nitrogen utilization; post-embryonic camera-type eye morphogenesis; regulation of protein heterodimerization activity; positive regulation of pigmentation; apoptosis; T cell homeostatic proliferation; neuron migration; positive regulation of apoptosis involved in mammary gland involution; negative regulation of peptidyl-serine phosphorylation; response to salt stress; release of matrix enzymes from mitochondria; positive regulation of protein oligomerization; apoptotic mitochondrial changes; B cell homeostatic proliferation; ovarian follicle development; B cell homeostasis; positive regulation of neuron apoptosis; response to gamma radiation; B cell negative selection; response to axon injury; protein homooligomerization; caspase activation; transformed cell apoptosis; mitochondrial fusion; Sertoli cell proliferation; odontogenesis of dentine-containing teeth; endoplasmic reticulum calcium ion homeostasis; neuron apoptosis; homeostasis of number of cells within a tissue; retinal cell programmed cell death; blood vessel remodeling; positive regulation of release of sequestered calcium ion into cytosol; caspase activation via cytochrome c