Rabbit IRX3 Polyclonal Antibody | anti-IRX3 antibody

IRX3 Polyclonal Antibody

Cat. Num. AAA3007190

• Gene Names: IRX3; IRX-1; IRXB1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IRX3; Polyclonal Antibody; IRX3 Polyclonal Antibody; Iroquois-class homeodomain protein IRX-3; Homeodomain protein IRXB1; Iroquois homeobox protein 3; IRXB1; anti-IRX3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IRX3 polyclonal antibody detects endogenous levels of IRX3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 501

• Applicable Applications for anti-IRX3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human IRX3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of IRX3 polyclonal antibody at 1:500 dilution Lane1:RAW264. 7 whole cell lysate Lane2:PC12 whole cell lysateLane3:HELA whole cell lysate)

Related Product Information for anti-IRX3 antibody

The Iroquois homeobox gene family of transcription factors regulate aspects of embryonic development including anterior/posterior and dorsal/ventral axis patterning in the central nervous system. The Iroquois family are clustered on two loci, IRXA and IRXB, which map to chromosomes 8 and 13 in mice. The IRXA group includes IRX, IRX2 and IRX4; the IRXB group comprises IRX3, IRX5 and IRX6. IRX1 and IRX2 are both widely expressed during development in the lung epithelium and also in the ventricular septum. IRX1 and IRX2 also play a role in digit formation (E11.5-E14.5). The IRX gene family members are each expressed in a distinct pattern during mouse heart development. Specifically, IRX1 and IRX2 are expressed in the ventricular septum and IRX3 is expressed in the ventricular trabeculated myocardium. In addition, IRX4 is expressed in the linear heart tube and the AV canal; IRX5 is expressed in the endocardium lining the ventricular and atrial myocardium. Furthermore, the IRX4 gene may modulate cardiac development and function. Although the heart of IRX4(-) mice appears to develop normally, adult IRX4(-) mice exhibit cardiomyopathy, including cardiac hypertrophy and decreased contractility.

NCBI and Uniprot Product Information

• NCBI GI #: 226371735
• NCBI GeneID: 79191
• NCBI Accession #: NP_077312.2
• NCBI GenBank Nucleotide #: NP_077312.2
• UniProt Accession #: P78415
• Molecular Weight: ~ 52kDa
• NCBI Official Full Name: iroquois-class homeodomain protein IRX-3
• NCBI Official Synonym Full Names: iroquois homeobox 3
• NCBI Official Symbol: IRX3
• NCBI Official Synonym Symbols: IRX-1; IRXB1
• NCBI Protein Information: iroquois-class homeodomain protein IRX-3
• UniProt Protein Name: Iroquois-class homeodomain protein IRX-3
• Protein Family: Iroquois-class homeodomain protein
• UniProt Gene Name: IRX3
• UniProt Synonym Gene Names: IRXB1
• UniProt Entry Name: IRX3_HUMAN

NCBI Description

IRX3 is a member of the Iroquois homeobox gene family (see IRX1; MIM 606197) and plays a role in an early step of neural development (Bellefroid et al., 1998 [PubMed 9427753]). Members of this family appear to play multiple roles during pattern formation of vertebrate embryos (Lewis et al., 1999 [PubMed 10370142]).[supplied by OMIM, Aug 2009]

Uniprot Description

IRX3: Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells. Belongs to the TALE/IRO homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: axon; cytoplasm; nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; mesoderm development; positive regulation of neuron differentiation; metanephros development