Rabbit RAD1 Polyclonal Antibody | anti-RAD1 antibody

RAD1 Polyclonal Antibody

Cat. Num. AAA3006516

• Gene Names: RAD1; REC1; HRAD1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RAD1; Polyclonal Antibody; RAD1 Polyclonal Antibody; Cell cycle checkpoint protein RAD1; hRAD1; DNA repair exonuclease rad1 homolog; Rad1-like DNA damage checkpoint protein; REC1; anti-RAD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RAD1 polyclonal antibody detects endogenous levels of RAD1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 282

• Applicable Applications for anti-RAD1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human RAD1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RAD1 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)

Related Product Information for anti-RAD1 antibody

DNA damage or incomplete replication of DNA results in inhibition of cell cycle progression at the G1-S or G2-M checkpoints by conserved regulatory mechanisms. Rad17 is involved in regulation of cell cycle arrest at the G1 checkpoint, whereas Chk1, Rad1, Rad9 and Hus1 are involved in regulation of cell cycle arrest at the G2 checkpoint. Over- expression of Rad17 results in p53 activation and an accumulation of cells in G1 phase. Chk1 functions as an essential component in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage, thus inhibiting mitosis. Hus1 and Rad9 exhibit conserved function in fission yeast and higher eukaryotes. Hus1 has been shown to be phosphorylated in response to DNA damage, a process which requires rad checkpoint genes. Rad9 is thought to be a candidate tumor sup-pressor gene because it is localized to a region of human chromosome 11 containing a number of tumor suppressor loci.

NCBI and Uniprot Product Information

• NCBI GI #: 74735450
• NCBI GeneID: 5810
• UniProt Accession #: O60671
• Molecular Weight: ~ 32kDa
• NCBI Official Full Name: Cell cycle checkpoint protein RAD1
• NCBI Official Synonym Full Names: RAD1 checkpoint DNA exonuclease
• NCBI Official Symbol: RAD1
• NCBI Official Synonym Symbols: REC1; HRAD1
• NCBI Protein Information: cell cycle checkpoint protein RAD1
• UniProt Protein Name: Cell cycle checkpoint protein RAD1
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD1
• UniProt Synonym Gene Names: REC1; hRAD1
• UniProt Entry Name: RAD1_HUMAN

NCBI Description

This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

RAD1: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity. Belongs to the rad1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; DNA repair, damage; Deoxyribonuclease; EC 3.1.11.2

Chromosomal Location of Human Ortholog: 5p13.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; chromosome; nucleus

Molecular Function: protein binding; exodeoxyribonuclease III activity; damaged DNA binding; 3'-5' exonuclease activity

Biological Process: meiotic recombination checkpoint; substantia nigra development; DNA damage checkpoint; DNA repair; DNA catabolic process, exonucleolytic; DNA replication; response to DNA damage stimulus