Rabbit ARHGEF5 Polyclonal Antibody | anti-ARHGEF5 antibody

ARHGEF5 Polyclonal Antibody

Cat. Num. AAA3006055

• Gene Names: ARHGEF5; P60; TIM; GEF5; TIM1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ARHGEF5; Polyclonal Antibody; ARHGEF5 Polyclonal Antibody; Rho guanine nucleotide exchange factor 5; Ephexin-3; Ephexin 3; Ephexin3; Guanine nucleotide regulatory protein TIM; Oncogene TIM; Transforming immortalized mammary oncogene; p60 TIM; TIM; anti-ARHGEF5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ARHGEF5 polyclonal antibody detects endogenous levels of ARHGEF5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1597

• Applicable Applications for anti-ARHGEF5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 1310-1355 of Human ARHGEF5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ARHGEF5 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-ARHGEF5 antibody

Rho GTPases, which play fundamental roles in numerous cellular processes, are initiated by external stimuli that signal though G-protein coupled receptors. ARHGEF5 (Rho guanine nucleotide exchange factor (GEF) 5), also known as P60 or transforming immortalized mammary oncogene (TIM), is a 1597 amino acid cytoplasmic protein. ARHGEF5 is believed to form a complex with G proteins and be involved in the initiation of Rho-dependent signals. It has also been suggested that ARHGEF5 may be involved in the organization of the cytoskeleton. ARHGEF5 contains one PH domain, one DH domain and one SH3 domain. Existing as two isoforms produced by alternative splicing, ARHGEF5 is mainly expressed in pancreas, liver, kidney, placenta and lung.

NCBI and Uniprot Product Information

• NCBI GI #: 50959191
• NCBI GeneID: 7984
• NCBI Accession #: NP_005426.2
• NCBI GenBank Nucleotide #: NP_005426.2
• UniProt Accession #: Q12774
• Molecular Weight: ~ 60, 177kDa
• NCBI Official Full Name: rho guanine nucleotide exchange factor 5
• NCBI Official Synonym Full Names: Rho guanine nucleotide exchange factor 5
• NCBI Official Symbol: ARHGEF5
• NCBI Official Synonym Symbols: P60; TIM; GEF5; TIM1
• NCBI Protein Information: rho guanine nucleotide exchange factor 5
• UniProt Protein Name: Rho guanine nucleotide exchange factor 5
• Protein Family: Rho guanine nucleotide exchange factor
• UniProt Gene Name: ARHGEF5
• UniProt Synonym Gene Names: TIM
• UniProt Entry Name: ARHG5_HUMAN

NCBI Description

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008]

Uniprot Description

ARHGEF5: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; Oncoprotein; Motility/polarity/chemotaxis; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 7q35

Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; GTP binding

Biological Process: myeloid dendritic cell chemotaxis; actin cytoskeleton organization and biogenesis