Rabbit LHX1 Polyclonal Antibody | anti-LHX1 antibody

LHX1 Polyclonal Antibody

Cat. Num. AAA3005958

• Gene Names: LHX1; LIM1; LIM-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: LHX1; Polyclonal Antibody; LHX1 Polyclonal Antibody; LIM/homeobox protein Lhx1; LIM homeobox protein 1; Homeobox protein Lim-1; hLim-1; LIM-1; LIM1; LHX-1; anti-LHX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LHX1 polyclonal antibody detects endogenous levels of LHX1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 406

• Applicable Applications for anti-LHX1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; ICC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the 186-231 of Human LHX1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of LHX1 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-LHX1 antibody

During development, genetically distinct subtypes of motor neurons express unique combinations of LIM-type homeodomain factors, which regulate cell migration and guide motor axons to establish the fidelity of a binary choice in axonal trajectory. The LIM gene family encodes a set of gene products, which carry the LIM domain, a unique cysteine-rich zinc-binding domain. At least 40 members of this family have been identified in vertebrates and invertebrates, and are distributed into 4 groups according to the number of LIM domains and to the presence of homeodomains and kinase domains. The overlapping expression of LHX1, LHX3, LHX4, Isl-1 and Isl-2 in developing motorneurons along the spinal column may influence the establishment of specific motorneuron subtypes. The human LHX1 gene maps to chromosome 11p13-p12 and encodes a 384 amino acid protein. The human LHX1 transcript is assembled from five exons, which are separated by introns ranging in size from 93 nt to 2.3 kb. The two LIM domains are entirely contained in the first and second exons, respectively, while the homeodomain is split into exons three and four.

NCBI and Uniprot Product Information

• NCBI GI #: 30410785
• NCBI GeneID: 3975
• NCBI Accession #: NP_005559.2
• NCBI GenBank Nucleotide #: NP_005559.2
• UniProt Accession #: P48742
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: LIM/homeobox protein Lhx1
• NCBI Official Synonym Full Names: LIM homeobox 1
• NCBI Official Symbol: LHX1
• NCBI Official Synonym Symbols: LIM1; LIM-1
• NCBI Protein Information: LIM/homeobox protein Lhx1
• UniProt Protein Name: LIM/homeobox protein Lhx1
• UniProt Gene Name: LHX1
• UniProt Synonym Gene Names: LIM-1; LIM1; LIM homeobox protein 1; hLim-1
• UniProt Entry Name: LHX1_HUMAN

NCBI Description

This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract. [provided by RefSeq, Dec 2010]

Uniprot Description

LHX1: Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.

Protein type: Transcription factor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: protein complex; nucleus

Molecular Function: zinc ion binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; motor axon guidance; uterus development; forebrain regionalization; embryonic pattern specification; pronephros development; post-embryonic development; anterior/posterior pattern formation; cell-cell signaling; ectoderm formation; ureteric bud development; anatomical structure formation; cerebellum development; kidney development; fallopian tube development; cervix development; nervous system development; anatomical structure morphogenesis; vagina development; ventral spinal cord development; gastrulation with mouth forming second; embryonic viscerocranium morphogenesis; somite rostral/caudal axis specification; pattern specification process; Purkinje cell-granule cell precursor cell signaling involved in regulation of granule cell precursor cell proliferation; organ morphogenesis; telencephalon development; positive regulation of embryonic development; dorsal/ventral pattern formation; regulation of gene expression; endoderm formation; ureteric bud branching; embryonic retina morphogenesis in camera-type eye; cerebellar Purkinje cell differentiation; spinal cord association neuron differentiation; negative regulation of transcription, DNA-dependent; urogenital system development; anterior/posterior axis specification