Rabbit JAK1 Polyclonal Antibody | anti-JAK1 antibody

JAK1 (Phospho-Y1022) Polyclonal Antibody

Cat. Num. AAA3004678

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: JAK1; Polyclonal Antibody; JAK1 (Phospho-Y1022) Polyclonal Antibody; Tyrosine-protein kinase JAK1; Janus kinase 1; JAK-1; JAK1A; JAK1B; anti-JAK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-JAK1 (Y1022) polyclonal antibody detects endogenous levels of JAK1 protein only when phosphorylated at Tyr1022.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-JAK1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human JAK1 around the phosphorylation site of Tyrosine 1022.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-JAK1 (Y1022) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-JAK1 antibody

JAK1 (Janus kinase 1) belongs to the family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK1. JAK1 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. Upon ligand binding, JAK1 undergoes tyrosine phosphorylation and catalytic activation in an interdependent manner. Phosphorylation of tyrosine residues at positions 1022 and 1023 is believed to function in the activation of catalytic events.

NCBI and Uniprot Product Information

• UniProt Accession #: P23458
• Molecular Weight: ~ 132kDa
• UniProt Protein Name: Tyrosine-protein kinase JAK1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: JAK1
• UniProt Synonym Gene Names: JAK1A; JAK1B; JAK-1
• UniProt Entry Name: JAK1_HUMAN

Uniprot Description

JAK1: a widely expressed non-receptor tyrosine-kinase involved in the interferon-alpha/beta and -gamma signal transduction pathways. Couples cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. A selective Jak1 inhibitor induces apoptosis in NRP-154 prostate cancer cell line. A single mutation seen in tyrosine kinome screen of colon cancers. Activated in B cell lines from patients with post-transplant lymphoproliferative disorder, and in a mouse Lck-driven T cell lymphoma. Inhibitor: Piceatannol.

Protein type: EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; JakA family

Chromosomal Location of Human Ortholog: 1p32.3-p31.3

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; focal adhesion; cytoplasm; endomembrane system; nucleus; cytosol

Molecular Function: protein binding; growth hormone receptor binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; CCR5 chemokine receptor binding

Biological Process: response to antibiotic; cell migration; cytokine and chemokine mediated signaling pathway; innate immune response; cell differentiation; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation