p-G3BP-1 blocking peptide

p-G3BP-1 (S232) Peptide

Cat. Num. AAA3004627

• Applications: Blocking
• Purity: Synthetic peptide p-G3BP-1 (S232). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Synonyms: p-G3BP-1; p-G3BP-1 (S232) Peptide; G3BP1; G3BP; hDH VIII; G3BP-1; Ras GTPase-activating protein-binding protein 1; GAP SH3 domain-binding protein 1; p-G3BP-1 blocking peptide

• Specificity: This peptide can be used with studies using MBS3004626 p-G3BP-1 (S232) pAb.
• Purity/Purification: Synthetic peptide p-G3BP-1 (S232). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Form/Format: 1 mg/ml in DI water.
• Sequence Length: 466

• Applicable Applications for p-G3BP-1 blocking peptide: Blocking
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Related Product Information for p-G3BP-1 blocking peptide

G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 5031703
• NCBI Accession #: NP_005745.1
• NCBI GenBank Nucleotide #: NP_005745.1
• UniProt Accession #: Q13283
• NCBI Official Full Name: ras GTPase-activating protein-binding protein 1
• UniProt Protein Name: Ras GTPase-activating protein-binding protein 1
• UniProt Gene Name: G3BP1
• UniProt Synonym Gene Names: G3BP; G3BP-1; hDH VIII
• UniProt Entry Name: G3BP1_HUMAN

Uniprot Description

G3BP-1: an hnRNA-binding protein and endoribonuclease that participates in the Ras signal transduction pathway. A regulated effector of stress granule (SG) assembly. SGs are involved in mRNA sorting in the storing of untranslated mRNAs. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Binds to the SH3 domain of Ras GTPase-activating protein (RasGAP) in proliferating cells but not in quiescent cells. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells. Cytosolic and partially nuclear in resting cells. Recruited to SGs upon either arsenite or high temperature treatment. RasGAP-dependent phosphorylation of S149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. S149 phosphorylation induces partial nuclear localization. A component of a TAU mRNP complex, Interacts with USP10, and may regulate it.

Protein type: EC 3.6.4.12; RNA-binding; Helicase; Adaptor/scaffold; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: focal adhesion; stress granule; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: mRNA binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; endonuclease activity; ATP-dependent RNA helicase activity; ATP binding

Biological Process: transport; metabolic process; Ras protein signal transduction; DNA duplex unwinding