Rabbit PAK2 Polyclonal Antibody | anti-PAK2 antibody

PAK2 (R186) Polyclonal Antibody

Cat. Num. AAA3004249

• Gene Names: PAK2; PAK65; PAKgamma
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PAK2; Polyclonal Antibody; PAK2 (R186) Polyclonal Antibody; Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58; PAK-2p27; PAK-2p34; C-t-PAK2; PAKgamma; anti-PAK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PAK2 (R186) polyclonal antibody detects endogenous levels of PAK2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 524

• Applicable Applications for anti-PAK2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 150-200 of Human PAK2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PAK2 (R186) polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:The spleen tissue lysate of Mouse (40ug) Lane3:The spleen tissue lysate of Rat (40ug) Lane4:SK-OVCAR3 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PAK2 (R186) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)

Related Product Information for anti-PAK2 antibody

Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

NCBI and Uniprot Product Information

• NCBI GI #: 32483399
• NCBI GeneID: 5062
• NCBI Accession #: NP_002568.2
• NCBI GenBank Nucleotide #: NP_002568.2
• UniProt Accession #: Q13177
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: serine/threonine-protein kinase PAK 2
• NCBI Official Synonym Full Names: p21 (RAC1) activated kinase 2
• NCBI Official Symbol: PAK2
• NCBI Official Synonym Symbols: PAK65; PAKgamma
• NCBI Protein Information: serine/threonine-protein kinase PAK 2
• UniProt Protein Name: Serine/threonine-protein kinase PAK 2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK2
• UniProt Synonym Gene Names: PAK-2; p27; p34
• UniProt Entry Name: PAK2_HUMAN

NCBI Description

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008]

Uniprot Description

PAK2: a protein kinase of the STE20 family. Critical effector linking RhoGTPases to cytoskeleton reorganization and nuclear signaling. Activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.

Protein type: Protein kinase, STE; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKA subfamily

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; protein tyrosine kinase activator activity; protein kinase binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: axon guidance; viral reproduction; apoptosis; protein amino acid autophosphorylation; signal transduction; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of defense response to virus by virus; regulation of apoptosis; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of growth; T cell costimulation; innate immune response; negative regulation of protein kinase activity; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; phosphorylation; negative regulation of apoptosis; cell structure disassembly during apoptosis