Rabbit TIS11B Polyclonal Antibody | anti-TIS11B antibody

TIS11B (D88) Polyclonal Antibody

Cat. Num. AAA3003303

• Gene Names: ZFP36L1; BRF1; ERF1; cMG1; ERF-1; Berg36; TIS11B; RNF162B
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TIS11B; Polyclonal Antibody; TIS11B (D88) Polyclonal Antibody; Zinc finger protein 36; C3H1 type-like 1; Butyrate response factor 1; EGF-response factor 1; ERF-1; Protein TIS11B; ZFP36L1; BERG36; BRF1; ERF1; RNF162B; anti-TIS11B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TIS11B (D88) polyclonal antibody detects endogenous levels of TIS11B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 338

• Applicable Applications for anti-TIS11B antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human TIS11B.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TIS11B (D88) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)

Related Product Information for anti-TIS11B antibody

TIS11B (tetradecanoyl phorbol acetate-inducible sequence 11b), also known as ZFP36L1, BRF1 (butyrate response factor 1), ERF1 (EGF response factor 1), cMG1, Berg36 or RNF162B, is a member of the tristetraprolin family. Tristetraprolin (TTP), or TIS11, is a zinc-binding protein encoded by the immediate-early response gene, Zfp-36. TIS11B, a relative of TTP, localizes to the nucleus and may function as a transcription factor involved in regulating the growth factor response. It is an evolutionarily conserved protein containing two C3H1-type zinc fingers and a repeating Cys-His motif. TIS11B is an mRNA binding protein and is known to interact with the 3'-untranslated region of VEGF mRNA, thereby decreasing its stability. This suggests that TIS11B is a potential target in antiangiogenic therapy. In addition, TIS11B may also be an important regulator of myogenesis, as its expression is upregulated during murine myoblast differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 347659004
• NCBI GeneID: 677
• NCBI Accession #: NP_001231627.1
• NCBI GenBank Nucleotide #: NP_001231627.1
• UniProt Accession #: Q07352
• Molecular Weight: ~ 36kDa
• NCBI Official Full Name: mRNA decay activator protein ZFP36L1 isoform 1
• NCBI Official Synonym Full Names: ZFP36 ring finger protein like 1
• NCBI Official Symbol: ZFP36L1
• NCBI Official Synonym Symbols: BRF1; ERF1; cMG1; ERF-1; Berg36; TIS11B; RNF162B
• NCBI Protein Information: mRNA decay activator protein ZFP36L1
• UniProt Protein Name: Zinc finger protein 36, C3H1 type-like 1
• UniProt Gene Name: ZFP36L1
• UniProt Synonym Gene Names: BERG36; BRF1; ERF1; RNF162B; TIS11B; ERF-1
• UniProt Entry Name: TISB_HUMAN

NCBI Description

This gene is a member of the TIS11 family of early response genes, which are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. This gene is well conserved across species and has a promoter that contains motifs seen in other early-response genes. The encoded protein contains a distinguishing putative zinc finger domain with a repeating cys-his motif. This putative nuclear transcription factor most likely functions in regulating the response to growth factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

BRF1: a zinc finger protein that plays an important role in the assembly of the RNA polymerase III initiation factor TFIIIB. Regulates mRNA levels by targeting transcripts containing AREs (AU-rich elements) into the decay pathway. Phosphorylation by Akt apparently generates 14-3-3 binding sites and inhibits BRF1 from promoting mRNA activity.Contains 2 C3H1-type zinc fingers.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 14q22-q24

Cellular Component: nucleus; cytosol

Molecular Function: mRNA binding; protein binding; DNA binding; metal ion binding; transcription factor activity

Biological Process: regulation of translation; neural tube development; cell proliferation; regulation of transcription, DNA-dependent; apoptosis; heart development; multicellular organism growth; T cell differentiation in the thymus; gene expression; regulation of mRNA stability; vasculogenesis; mRNA catabolic process, deadenylation-dependent decay