Rabbit GPR10 Polyclonal Antibody | anti-GPR10 antibody

GPR10 (Q223) Polyclonal Antibody

Cat. Num. AAA3003237

• Gene Names: PRLHR; GR3; GPR10; PrRPR
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GPR10; Polyclonal Antibody; GPR10 (Q223) Polyclonal Antibody; Prolactin-releasing peptide receptor; PrRP receptor; PrRPR; G-protein coupled receptor 10; hGR3; PRLHR; GR3; anti-GPR10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GPR10 (Q223) polyclonal antibody detects endogenous levels of GPR10 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 370

• Applicable Applications for anti-GPR10 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 200-250 of Human GPR10.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GPR10 (Q223) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Hela whole cell lysateLane3:Rat brain tissue lysate)

Related Product Information for anti-GPR10 antibody

G protein-coupled receptors (GPRs or GPCRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, are members of the largest protein family and play a role in many different stimulus-response pathways. G protein-coupled receptors mediate extracellular signals into intracellular signals (G protein activation). They respond to a great variety of signaling molecules, including hormones, neurotransmitters and other proteins and peptides. GPR proteins are integral seven-pass membrane proteins with some conserved amino acid regions. G protein coupled receptor 10 (GPR10) acts as a receptor for prolactin-releasing peptide (PrRP). GPR10 plays a role in the regulation of food intake, pain-signal processing and in lactation. Primarily expressed in pituitary gland, it is repressed by bromocriptine. GPR10 interacts with various other proteins, including GRIP1, GRIP2 and PICK1.

NCBI and Uniprot Product Information

• NCBI GI #: 4758474
• NCBI GeneID: 2834
• NCBI Accession #: NP_004239.1
• NCBI GenBank Nucleotide #: NP_004239.1
• UniProt Accession #: P49683
• Molecular Weight: ~ 41kDa
• NCBI Official Full Name: prolactin-releasing peptide receptor
• NCBI Official Synonym Full Names: prolactin releasing hormone receptor
• NCBI Official Symbol: PRLHR
• NCBI Official Synonym Symbols: GR3; GPR10; PrRPR
• NCBI Protein Information: prolactin-releasing peptide receptor
• UniProt Protein Name: Prolactin-releasing peptide receptor
• UniProt Gene Name: PRLHR
• UniProt Synonym Gene Names: GPR10; GR3; PrRP receptor; PrRPR
• UniProt Entry Name: PRLHR_HUMAN

NCBI Description

PRLHR is a 7-transmembrane domain receptor for prolactin-releasing hormone (PRLH; MIM 602663) that is highly expressed in anterior pituitary (Ozawa et al., 2002 [PubMed 11923475]).[supplied by OMIM, Mar 2008]

Uniprot Description

GPR10: Receptor for prolactin-releasing peptide (PrRP). Implicated in lactation, regulation of food intake and pain-signal processing. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26.13

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; neuropeptide receptor activity; neuropeptide Y receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; neuropeptide signaling pathway; hormone metabolic process; feeding behavior; female pregnancy