Rabbit CRF-RI Polyclonal Antibody | anti-CRF-RI antibody

CRF-RI (H177) Polyclonal Antibody

Cat. Num. AAA3002816

• Gene Names: CRHR1; CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CRF-RI; Polyclonal Antibody; CRF-RI (H177) Polyclonal Antibody; Corticotropin-releasing factor receptor 1; CRF-R-1; CRF-R1; CRFR-1; Corticotropin-releasing hormone receptor 1; CRH-R-1; CRH-R1; CRHR1; CRFR; CRFR1; CRHR; anti-CRF-RI antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CRF-RI (H177) polyclonal antibody detects endogenous levels of CRF-RI protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 444

• Applicable Applications for anti-CRF-RI antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 150-200 of Human CRF-RI.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CRF-RI (H177) pAb at 1:1000 dilutionLane1:L02 whole cell lysate (20ug) Lane2:MCF-7 whole cell lysate (20ug) Lane3:A549 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of CRF-RI (H105) pAb at 1:500 dilutionLane1:Jurkat whole cell lysate (40ug) Lane2:THP-1 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug))

Related Product Information for anti-CRF-RI antibody

A high affinity CRF binding protein, designated CRF-BP, has been discovered in postmortem brain samples from AD patients. CRF-BP serves to bind and inactivate CRF, reducing the pool of "free CRF" available to bind CRFRs. Two CRF receptors, designated CRF-RI and CFR-RII, exhibit distinct brain localizations.Two forms of CFR-RII, designated CFR-RIIalpha and CFR-RIIbeta, result from alternative mRNA splicing. Urocortin, an additional member of the CRF family, shares 63% sequence identity with urotensin and 45% sequence identity with CRF. Urocortin specifically binds to and activates CRF-RI and CRF-RII, but binds to CRF-RII more efficiently than CRF, suggesting that it may be the true, high affinity ligand for the CRF receptor type II.

NCBI and Uniprot Product Information

• NCBI GI #: 223717971
• NCBI GeneID: 1394
• NCBI Accession #: NP_001138618.1
• NCBI GenBank Nucleotide #: NP_001138618.1
• UniProt Accession #: P34998
• Molecular Weight: ~ 50kDa
• NCBI Official Full Name: corticotropin-releasing factor receptor 1 isoform 1
• NCBI Official Synonym Full Names: corticotropin releasing hormone receptor 1
• NCBI Official Symbol: CRHR1
• NCBI Official Synonym Symbols: CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1
• NCBI Protein Information: corticotropin-releasing factor receptor 1
• UniProt Protein Name: Corticotropin-releasing factor receptor 1
• UniProt Gene Name: CRHR1
• UniProt Synonym Gene Names: CRFR; CRFR1; CRHR; CRF-R-1; CRF-R1; CRFR-1; CRH-R-1; CRH-R1
• UniProt Entry Name: CRFR1_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Naturally-occurring readthrough transcription between this gene and upstream GeneID:147081 results in transcripts that encode isoforms that share similarity with the products of this gene. [provided by RefSeq, Aug 2016]

Uniprot Description

CRF-R1: a G-protein coupled receptor that specifically binds corticotropin-releasing hormone, a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress. Four splice-variant isoforms have been described.

Protein type: Membrane protein, integral; GPCR, family 2; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: multivesicular body; cell soma; apical part of cell; integral to plasma membrane; dendrite; integral to membrane; plasma membrane; trans-Golgi network; vesicle; intrinsic to plasma membrane

Molecular Function: protein binding; corticotropin-releasing hormone binding; corticotropin-releasing hormone receptor activity; protein complex binding; G-protein alpha-subunit binding; corticotrophin-releasing factor receptor activity

Biological Process: fear response; hypothalamus development; hormone-mediated signaling; adrenal gland development; adenylate cyclase activation; positive regulation of mast cell degranulation; female pregnancy; behavioral response to ethanol; memory; general adaptation syndrome, behavioral process; elevation of cytosolic calcium ion concentration; behavioral response to pain; epithelial cell differentiation; neuropeptide signaling pathway; behavioral response to cocaine; adrenocorticotropin hormone secretion; response to hypoxia; negative regulation of epinephrine secretion; visual learning; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); immune response; parturition; response to electrical stimulus