Rabbit anti-Human DAN  Polyclonal Antibody | anti-DAN  antibody

DAN (T161) Polyclonal Antibody

Cat. Num. AAA3002668

• Gene Names: NBL1; NB; DAN; NO3; DAND1; D1S1733E
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DAN ; Polyclonal Antibody; DAN (T161) Polyclonal Antibody; Tumor protein D54; hD54; Tumor protein D52-like 2; TPD52L2; anti-DAN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DAN (T161) pAb detects endogenous levels of DAN protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 181

• Applicable Applications for anti-DAN antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 185-245 of Human TPD54.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DAN  (T161) pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug))

Testing Data

Related Product Information for anti-DAN  antibody

NBL1 (neuroblastoma suppression of tumorigenicity 1), also named as DAN, is the founding member of the DAN subfamily. It was originally identified as a putative tumor suppressor gene in a transformed fibroblast rat model (PMID: 9516839), whereby it was significantly down-regulated in v-src-transformed rat fibroblasts. The MW of this protein is 19 kDa, and this antibody specially recognises the 19 kDa protein.

NCBI and Uniprot Product Information

• NCBI GI #: 323276675
• NCBI GeneID: 4681
• NCBI Accession #: NP_001191013.1
• NCBI GenBank Nucleotide #: NP_001191013.1
• UniProt Accession #: P41271
• Molecular Weight: ~ 19kDa
• NCBI Official Full Name: neuroblastoma suppressor of tumorigenicity 1 isoform 2
• NCBI Official Synonym Full Names: NBL1, DAN family BMP antagonist
• NCBI Official Symbol: NBL1
• NCBI Official Synonym Symbols: NB; DAN; NO3; DAND1; D1S1733E
• NCBI Protein Information: neuroblastoma suppressor of tumorigenicity 1
• UniProt Protein Name: Neuroblastoma suppressor of tumorigenicity 1
• UniProt Gene Name: NBL1
• UniProt Synonym Gene Names: DAN; DAND1
• UniProt Entry Name: NBL1_HUMAN

NCBI Description

This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]

Uniprot Description

NBL1 iso2: Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Tumor suppressor; Secreted

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: extracellular space

Molecular Function: morphogen activity; protein homodimerization activity

Biological Process: nervous system development; determination of dorsal identity; positive regulation of neuron differentiation; neurite morphogenesis; negative regulation of BMP signaling pathway