Rabbit DAP-5 Polyclonal Antibody | anti-DAP-5 antibody

DAP-5 (N65) Polyclonal Antibody

Cat. Num. AAA3002576

• Gene Names: EIF4G2; P97; AAG1; DAP5; NAT1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DAP-5; Polyclonal Antibody; DAP-5 (N65) Polyclonal Antibody; Eukaryotic translation initiation factor 4 gamma 2; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; Death-associated protein 5; p97; EIF4G2; DAP5; OK/SW-cl.75; anti-DAP-5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DAP-5 (N65) polyclonal antibody detects endogenous levels of DAP-5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 869

• Applicable Applications for anti-DAP-5 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 35-80 of Human DAP-5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DAP-5 (N65) polyclonal antibody at 1:500 dilutionLane1:HepG2 cell lysate Lane2:Raw264. 7 cell lysateLane3:H9C2 cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of DAP-5 (N65) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of DAP-5 (N65) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of DAP-5 (N65) pAb in paraffin-embedded human stomach carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-DAP-5 antibody

Death-associated protein 5 (DAP-5) (also known as p97 and NAT1) is a member of the eukaryotic translation initiation factor 4G (elF4G) family. DAP-5 is ubiquitously expressed and is highly conserved among species. In response to activated FAS or p53, caspase cleaves DAP-5 at position 790 to yield a C-terminal truncated protein which is capable of forming complexes with eIF4A and eIF3. DAP-5 has homology to the carboxy-terminal portion of eIF4G, but lacks the N-terminal region of eIF4G, which is responsible for association with the CAP binding protein eIF4E. By forming translationally inactive complexes with eIF4A and eIF3, but not with eIF4E, DAP-5 functions as a general repressor of translation. During apotosis, the caspase-activated DAP-5 can mediate CAP-independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP-5. DAP-5 is also required for cellular differentiation, as it controls specific gene expression pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 289577114
• NCBI GeneID: 1982
• NCBI Accession #: NP_001036024.3
• NCBI GenBank Nucleotide #: NP_001036024.3
• UniProt Accession #: P78344
• Molecular Weight: ~ 90kDa
• NCBI Official Full Name: eukaryotic translation initiation factor 4 gamma 2 isoform 2
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4 gamma 2
• NCBI Official Symbol: EIF4G2
• NCBI Official Synonym Symbols: P97; AAG1; DAP5; NAT1
• NCBI Protein Information: eukaryotic translation initiation factor 4 gamma 2
• UniProt Protein Name: Eukaryotic translation initiation factor 4 gamma 2
• UniProt Gene Name: EIF4G2
• UniProt Synonym Gene Names: DAP5; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; DAP-5
• UniProt Entry Name: IF4G2_HUMAN

NCBI Description

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

EIF4G2: a general repressor of translation by forming translationally inactive complexes. Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Interacts with the protein kinases Mnk1 and -2. Binds EIF4A and EIF3. Cleaved by some caspases and viral proteases.

Protein type: Translation initiation; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: membrane; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity

Biological Process: cell death; cytokine and chemokine mediated signaling pathway; translational initiation; cell cycle arrest; regulation of translational initiation