Rabbit anti-Human MAP2K7 Polyclonal Antibody | anti-MAP2K7 antibody

MAP2K7 (Ab-271) polyclonal antibody

Cat. Num. AAA3001634

• Gene Names: MAP2K7; MKK7; Jnkk2; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: MAP2K7; Polyclonal Antibody; MAP2K7 (Ab-271) polyclonal antibody; JNKK2; MEK7; MKK7; PRKMK7; SKK4; Dual specificity mitogen-activated protein kinase kinase 7; MAP kinase kinase 7; MAPKK 7; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; Stress-activated protein kinase kinase 4; SAPK kinase 4; S; anti-MAP2K7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Phospho-MEK-7 (S271) Polyclonal Antibody detects endogenous levels of MEK-7 protein only when phosphorylated at S271.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-MAP2K7 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
• Application Notes: WB: 1/500-1/2000; IHC: 1/100-1/300; ELISA: 1/20,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human MAP2K7 around the phosphorylation site of Ser271. AA range: 236-285
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of 3T3 cells using Phospho-MEK-7 (S271) Polyclonal Antibody)

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP2K7 (Phospho-Ser271) Antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K7 (Phospho-Ser271) Antibody. The picture on the right is blocked with the phospho peptide.)

WB4

(Western Blot (WB))

Related Product Information for anti-MAP2K7 antibody

Background: mitogen-activated protein kinase kinase 7(MAP2K7) Homo sapiens. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

NCBI and Uniprot Product Information

• NCBI GI #: 21735544
• NCBI GeneID: 5609
• NCBI Accession #: NP_660186.1
• NCBI GenBank Nucleotide #: NM_145185.2
• UniProt Accession #: O14733; O14648; O14816; O60452; O60453; Q1PG43; Q8IY10; B2R9S5; D6W659
• Molecular Weight: 47,485 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 7
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 7
• NCBI Official Symbol: MAP2K7
• NCBI Official Synonym Symbols: MKK7; Jnkk2; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 7; MEK 7; JNKK 2; MAPKK 7; SAPK kinase 4; MAPK/ERK kinase 7; MAP kinase kinase 7; JNK-activating kinase 2; c-Jun N-terminal kinase kinase 2; stress-activated protein kinase kinase 4
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 7
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K7
• UniProt Synonym Gene Names: JNKK2; MEK7; MKK7; PRKMK7; SKK4; MAP kinase kinase 7; MAPKK 7; MEK 7; SAPK kinase 4; SAPKK-4; SAPKK4; JNK kinase 2; JNKK 2
• UniProt Entry Name: MP2K7_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined. [provided by RefSeq, Jul 2008]

Uniprot Description

MKK7: a dual-specificity protein kinase of the STE7 family. Activates JNK1 and -2 by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by proinflammatory cytokines and environmental stress. Three alternatively spliced isoforms have been reported.

Protein type: Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; Kinase, protein; STE group; STE7 family

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: cytoplasm; intracellular; cytosol; nucleus

Molecular Function: MAP kinase kinase activity; protein C-terminus binding; protein serine/threonine kinase activity; protein binding; enzyme binding; protein-tyrosine kinase activity; magnesium ion binding; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; protein kinase binding; protein phosphatase binding; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; response to osmotic stress; signal transduction; activation of JNK activity; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to heat; positive regulation of neuron apoptosis; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; response to UV