Rabbit NIFK Polyclonal Antibody | anti-NIFK antibody

NIFK (D229) Polyclonal Antibody

Cat. Num. AAA3001504

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NIFK; Polyclonal Antibody; NIFK (D229) Polyclonal Antibody; MKI67 FHA domain-interacting nucleolar phosphoprotein; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK; MKI67IP; NOPP34; anti-NIFK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NIFK (D229) polyclonal antibody detects endogenous levels of NIFK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 293

• Applicable Applications for anti-NIFK antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 196-245 of Human NIFK.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NIFK (D229) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of NIFK (D229) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of NIFK (D229) pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug))

Related Product Information for anti-NIFK antibody

The structural proteins for the complex metalloenzyme nitrogenase include NIFK, NIFD and NIFH. These proteins are all necessary for archaeal and bacterial nitrogen fixation. The NIFK gene encodes the b subunit of the nitrogenase molybdenum-iron (MoFe) tetramer. NIFK localizes to the nucleolus where it interactes with the fork-head associated domain of the proliferation marker protein Ki-67 in a mitosis-specific and phosphorylation-dependent manner. NIFK is widely expressed in adult tissues, suggesting other functions in addition to its interaction with Ki-67, which is only expressed in proliferating cells.

NCBI and Uniprot Product Information

• NCBI GI #: 222352111
• NCBI Accession #: NP_115766.3
• NCBI GenBank Nucleotide #: NP_115766.3
• UniProt Accession #: Q9BYG3
• Molecular Weight: ~ 34kDa
• NCBI Official Full Name: MKI67 FHA domain-interacting nucleolar phosphoprotein
• UniProt Protein Name: MKI67 FHA domain-interacting nucleolar phosphoprotein
• Protein Family: Nitrogenase molybdenum-iron protein
• UniProt Gene Name: MKI67IP
• UniProt Synonym Gene Names: NIFK; NOPP34; hNIFK
• UniProt Entry Name: MK67I_HUMAN

Uniprot Description

NIFK: a nucleolar protein of unknown function that interacts with the fork-head associated (FHA) domain of the proliferation marker protein Ki-67

Protein type: RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 2q14.3

Cellular Component: nucleoplasm; condensed nuclear chromosome; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: rRNA metabolic process; protein complex assembly; rRNA transcription