Rabbit JAK3 Polyclonal Antibody | anti-JAK3 antibody

JAK3 (I781) Polyclonal Antibody

Cat. Num. AAA3001492

• Gene Names: JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: JAK3; Polyclonal Antibody; JAK3 (I781) Polyclonal Antibody; Tyrosine-protein kinase JAK3; Janus kinase 3; JAK-3; Leukocyte janus kinase; L-JAK; anti-JAK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: JAK3 (I781) polyclonal antibody detects endogenous levels of JAK3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1124

• Applicable Applications for anti-JAK3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 750-800 of Human JAK3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of JAK3 (I781) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysateLane3:PC12 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of JAK3 (I781) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-JAK3 antibody

Jak3 (Janus Kinase 3) belongs to the family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK3. JAK3 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 47157315
• NCBI GeneID: 3718
• NCBI Accession #: NP_000206.2
• NCBI GenBank Nucleotide #: NP_000206.2
• UniProt Accession #: P52333
• Molecular Weight: ~ 125kDa
• NCBI Official Full Name: tyrosine-protein kinase JAK3
• NCBI Official Synonym Full Names: Janus kinase 3
• NCBI Official Symbol: JAK3
• NCBI Official Synonym Symbols: JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
• NCBI Protein Information: tyrosine-protein kinase JAK3
• UniProt Protein Name: Tyrosine-protein kinase JAK3
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: JAK3
• UniProt Synonym Gene Names: JAK-3; L-JAK
• UniProt Entry Name: JAK3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]

Uniprot Description

JAK3: a non-receptor tyrosine-kinase of the Jak family expressed predominantly in immune cells. Mediates signal transduction via the common gamma-chain of cytokine receptors, including IL-2, -4, -7, -9, and -15. Interacts with members of the STAT (signal transduction and activators of transcription) family. Interacts with STAM2 and SHB. Contains two protein kinase domains. Mutations that abrogate JAK3 function cause an autosomal SCID (severe combined immunodeficiency disease). Inhibitor: R017s for organ transplants. Three differentially spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.2; TK group; JakA family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; endomembrane system; cytosol

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; hormone receptor binding

Biological Process: negative regulation of interleukin-12 production; cell migration; peptidyl-tyrosine phosphorylation; negative regulation of T-helper 1 cell differentiation; negative regulation of interleukin-10 production; negative regulation of FasL biosynthetic process; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; regulation of apoptosis; negative regulation of dendritic cell cytokine production; tyrosine phosphorylation of STAT protein; B cell differentiation; innate immune response; erythrocyte differentiation; positive regulation of T cell proliferation; T cell homeostasis; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of T cell activation; transmembrane receptor protein tyrosine kinase signaling pathway; STAT protein nuclear translocation

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-negative