Western Blot (WB)
(Western Blot (WB) analysis of Histone H3 (R2) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:BV2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug))
Rabbit Histone H3 Polyclonal Antibody | anti-H3 antibody
Histone H3 (R2) Polyclonal Antibody
IHC: 1:50-1:200
IF: 1:50-1:200
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Histone H3 (R2) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:BV2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of Histone H3 (R2) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:BV2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug))
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Histone H3 (R2) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Histone H3 (R2) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Immunofluorescence (IF)
(IF image of MBS3001470 stained A549 cells. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Histone H3 (R2) # MBS3001470 (1:100) at 10ug/ml overnight at +4 degree C. The secondary antibody (Green) was Goat Anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Hoechst33342 was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(IF image of MBS3001470 stained A549 cells. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Histone H3 (R2) # MBS3001470 (1:100) at 10ug/ml overnight at +4 degree C. The secondary antibody (Green) was Goat Anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Hoechst33342 was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
Uniprot Description
H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 6p22.2
Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus
Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity
Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly; DNA methylation on cytosine
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Product Notes
The H3 hist1h3a (Catalog #AAA3001470) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3 (R2) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the H3 hist1h3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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