Rabbit TNNI3K Polyclonal Antibody | anti-TNNI3K antibody

TNNI3K polyclonal antibody

Cat. Num. AAA3000637

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: TNNI3K; Polyclonal Antibody; TNNI3K polyclonal antibody; CARK; Serine/threonine-protein kinase TNNI3K; Cardiac ankyrin repeat kinase; Cardiac troponin I-interacting kinase; TNNI3-interacting kinase; anti-TNNI3K antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: TNNI3K Polyclonal Antibody detects endogenous levels of TNNI3K protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-TNNI3K antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
• Application Notes: IHC: 1/100-1/300; ELISA: 1/10,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human TNNI3K. AA range: 301-350
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of Mouse-kidney lysis using TNNI3K antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TNNI3K Antibody. The picture on the right is blocked with the synthesized peptide.)

Related Product Information for anti-TNNI3K antibody

Background: TNNI3 interacting kinase(TNNI3K) Homo sapiens. This gene encodes a protein that belongs to the MAP kinase kinase kinase (MAPKKK) family of protein kinases. The protein contains ankyrin repeat, protein kinase and serine-rich domains and is thought to play a role in cardiac physiology. [provided by RefSeq, Sep 2012]

NCBI and Uniprot Product Information

• NCBI GI #: 163914396
• NCBI GeneID: 100526835
• NCBI Accession #: NP_001106279.1
• NCBI GenBank Nucleotide #: NM_001112808.2
• UniProt Accession #: Q59H18; Q17RN0; Q49AR1; Q6MZS9; Q9Y2V6
• Molecular Weight: 92,851 Da
• NCBI Official Full Name: FPGT-TNNI3K fusion protein isoform a
• NCBI Official Synonym Full Names: FPGT-TNNI3K readthrough
• NCBI Official Symbol: FPGT-TNNI3K
• NCBI Protein Information: FPGT-TNNI3K fusion protein
• UniProt Protein Name: Serine/threonine-protein kinase TNNI3K
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: TNNI3K
• UniProt Synonym Gene Names: CARK
• UniProt Entry Name: TNI3K_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription from the neighboring fucose-1-phosphate guanylyltransferase (FPGT) and TNNI3 interacting kinase (TNNI3K) genes. Alternative splicing results in multiple transcript variants that are composed of in-frame exons from each individual gene. [provided by RefSeq, Dec 2010]

Uniprot Description

TNNI3K: May play a role in cardiac physiology. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; TKL group; MLK family; HH498 subfamily

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein C-terminus binding; protein serine/threonine kinase activity; protein binding; troponin I binding; metal ion binding; ATP binding; protein kinase activity

Biological Process: regulation of heart rate; protein amino acid phosphorylation

Disease: Cardiac Conduction Disease With Or Without Dilated Cardiomyopathy