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ICC (Immunocytochemistry)
(ICC staining Prohibitin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit Prohibitin Monoclonal Antibody | anti-PHB antibody
Prohibitin Antibody
Gene Names
PHB; PHB1
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Prohibitin; Monoclonal Antibody; Prohibitin Antibody; Epididymis luminal protein 215 antibody; Epididymis secretory sperm binding protein Li 54e antibody; HEL 215 antibody; HEL S 54e antibody; PHB antibody; PHB_HUMAN antibody; PHB1 antibody; Prohibitin antibody; anti-PHB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Monoclonal
Clone Number
SR46-02
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PHB antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:200-1:500
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:200-1:500
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining Prohibitin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Prohibitin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Prohibitin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Prohibitin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Prohibitin on hybrid fish (crucian-carp) liver tissue lysate using anti-Prohibitin antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of Prohibitin on hybrid fish (crucian-carp) liver tissue lysate using anti-Prohibitin antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of Prohibitin on different lysates using anti-Prohibitin antibody at 1/1, 000 dilution. Positive control: Lane 1: 293 Lane 2: Jurkat Lane 3: Mouse kidney Lane 4: HepG2)
WB (Western Blot)
(Western blot analysis of Prohibitin on different lysates using anti-Prohibitin antibody at 1/1, 000 dilution. Positive control: Lane 1: 293 Lane 2: Jurkat Lane 3: Mouse kidney Lane 4: HepG2)
Related Product Information for anti-PHB antibody
Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and protein levels are high in G1, decline during the S phase, rise again in G2 and decline in M phase, which suggests that prohibitin controls the cell cycle by using both transcriptional and posttranslational mechanisms. Prohibitin is also a potential tumor suppressor protein that binds to retinoblastoma (Rb) and subsequently inhibits the activity of E2F family members in response to specific signaling cascades. Prohibitin 2 is a repressor of estrogen receptor activity, and is required for somatic and germline differentiation in the larval gonad during embryonic development. Mutations in the Prohibitin genes are correlated with breast cancer development and/or progressionin more than 80% of the cell lines analyzed.
Product Categories/Family for anti-PHB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
29,804 Da
NCBI Official Full Name
prohibitin
NCBI Official Synonym Full Names
prohibitin
NCBI Official Symbol
PHB
NCBI Official Synonym Symbols
PHB1
NCBI Protein Information
prohibitin
UniProt Protein Name
Prohibitin
UniProt Gene Name
PHB
UniProt Entry Name
PHB_HUMAN
Similar Products
Product Notes
The PHB phb (Catalog #AAA29994) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Prohibitin Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's Prohibitin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:200-1:500 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PHB phb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prohibitin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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