Mouse Interleukin 35 ELISA Kit | IL-35 elisa kit

Mouse Interleukin 35 ELISA Kit

Cat. Num. AAA29510

• Gene Names: Ilf3; NF90; NFAR; MBII-26; MPHOSPH4
• Reactivity: Mouse
• Synonyms: Interleukin 35; Mouse Interleukin 35 ELISA Kit; IL-35 elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse IL-35 and analogues was observed.
• Sequence Length: 908

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 6.25 pg/ml - 200 pg/ml.
• Sensitivity: 1.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated IL-35 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL-35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-35 and IL-35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for IL-35 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated IL-35 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL-35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-35 and IL-35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 472339106
• NCBI GeneID: 16201
• NCBI Accession #: NP_001264250.1
• NCBI GenBank Nucleotide #: NM_001277321.1
• UniProt Accession #: Q9Z1X4; Q80VD5; Q812A1; Q8BP80; Q8C2H8; Q8K588
• Molecular Weight: 96,021 Da
• NCBI Official Full Name: interleukin enhancer-binding factor 3 isoform 5
• NCBI Official Synonym Full Names: interleukin enhancer binding factor 3
• NCBI Official Symbol: Ilf3
• NCBI Official Synonym Symbols: NF90; NFAR; MBII-26; MPHOSPH4
• NCBI Protein Information: interleukin enhancer-binding factor 3; nuclear factor 90; M phase phosphoprotein 4; nuclear factor associated with dsRNA
• UniProt Protein Name: Interleukin enhancer-binding factor 3
• UniProt Gene Name: Ilf3
• UniProt Entry Name: ILF3_MOUSE

NCBI Description

The protein encoded by this gene contains two double-stranded RNA binding domains and functions in the post-transcriptional regulation of gene expression. It is a component of an RNA-protein complex that may be involved in mediating the export of messenger RNAs. Alternative splicing results in multiple transcript variants encoding distinct isoforms. These isoforms are grouped into two categories, NFAR-1 or NFAR-2, based on variation at the C-terminus. [provided by RefSeq, Mar 2013]

Uniprot Description

NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression . Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described.

Protein type: Transcription factor; DNA-binding; Nucleolus; RNA-binding

Cellular Component: nucleoplasm; membrane; mitochondrion; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus

Molecular Function: enzyme binding; DNA binding; RNA binding; double-stranded RNA binding

Biological Process: protein amino acid methylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of transcription, DNA-dependent; negative regulation of translation; negative regulation of transcription, DNA-dependent; defense response to virus; protein amino acid phosphorylation