TRIB3 / Tribbles Homolog 3 Recombinant Protein | TRIB3 recombinant protein

Human TRIB3 / Tribbles Homolog 3 Recombinant Protein

• Gene Names: TRIB3; NIPK; SINK; TRB3; SKIP3; C20orf97
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: TRIB3 / Tribbles Homolog 3; Human TRIB3 / Tribbles Homolog 3 Recombinant Protein; Tribbles homolog 3; TRB-3; Neuronal cell death-inducible putative kinase; SINK; p65-interacting inhibitor of NF-kappa-B; C20orf97; NIPK; SKIP3; TRB3; TRIB3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 358

• Applicable Applications for TRIB3 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with AKT1, AKT2, ATF4, MAP2K1 and MAP2K7. Interacts with DDIT3/CHOP and inhibits its interaction with EP300/P300. Interacts (via N-terminus) with APOBEC3A. Interacts with APOBEC3C.
• Entry Function: Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. Can inhibit APOBEC3A editing of nuclear DNA.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 28201830
• NCBI GeneID: 57761
• UniProt Accession #: Q96RU7
• NCBI Official Full Name: Tribbles homolog 3
• NCBI Official Synonym Full Names: tribbles pseudokinase 3
• NCBI Official Symbol: TRIB3
• NCBI Official Synonym Symbols: NIPK; SINK; TRB3; SKIP3; C20orf97
• NCBI Protein Information: tribbles homolog 3
• UniProt Protein Name: Tribbles homolog 3
• Protein Family: Tribbles
• UniProt Gene Name: TRIB3
• UniProt Synonym Gene Names: C20orf97; NIPK; SKIP3; TRB3; TRB-3
• UniProt Entry Name: TRIB3_HUMAN

NCBI Description

The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TRB3: a Ca(2)/calmodulin-dependent protein kinase of the Trbl family. Induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. May negatively regulate the cell survival kinase AKT1.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 20p13-p12.2

Cellular Component: nucleoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; protein kinase inhibitor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity; kinase activity; transcription corepressor activity; protein kinase binding; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; nerve growth factor receptor signaling pathway; positive regulation of protein binding; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; protein amino acid phosphorylation; cellular response to insulin stimulus; regulation of MAP kinase activity; positive regulation of ubiquitin-protein ligase activity; insulin receptor signaling pathway; negative regulation of protein kinase activity; innate immune response; negative regulation of transcription, DNA-dependent; negative regulation of fatty acid biosynthetic process