SPN / Leukosialin Recombinant Protein | SPN recombinant protein

Human SPN / Leukosialin Recombinant Protein

• Gene Names: SPN; LSN; CD43; GALGP; GPL115
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: SPN / Leukosialin; Human SPN / Leukosialin Recombinant Protein; Leukosialin; Galactoglycoprotein; GALGP; Leukocyte sialoglycoprotein; Sialophorin; CD43; SPN recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 400

• Applicable Applications for SPN recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX.
• Entry Function: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 71979937
• NCBI GeneID: 6693
• NCBI Accession #: NP_001025459.1
• NCBI GenBank Nucleotide #: NP_001025459.1
• UniProt Accession #: P16150
• NCBI Official Full Name: leukosialin
• NCBI Official Synonym Full Names: sialophorin
• NCBI Official Symbol: SPN
• NCBI Official Synonym Symbols: LSN; CD43; GALGP; GPL115
• NCBI Protein Information: leukosialin
• UniProt Protein Name: Leukosialin
• Protein Family: Leukosialin
• UniProt Gene Name: SPN
• UniProt Synonym Gene Names: CD43; GALGP
• UniProt Entry Name: LEUK_HUMAN

NCBI Description

This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]

Uniprot Description

sialophorin: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX. Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; plasma membrane; basement membrane; uropod; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; regulation of defense response to virus; establishment and/or maintenance of cell polarity; negative regulation of cell adhesion; signal transduction; chemotaxis; negative thymic T cell selection; response to protozoan; negative regulation of T cell proliferation; cell surface receptor linked signal transduction; T cell costimulation; defense response to bacterium; cellular defense response; positive regulation of T cell proliferation; immune response; positive regulation of protein amino acid phosphorylation; negative regulation of type IV hypersensitivity; blood coagulation; leukocyte migration