CIB1 / Calcium And integrin-Binding Protein 1 Recombinant Protein | CIB1 recombinant protein

Human CIB1 / Calcium And integrin-Binding Protein 1 Recombinant Protein

• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: CIB1 / Calcium And integrin-Binding Protein 1; Human CIB1 / Calcium And integrin-Binding Protein 1 Recombinant Protein; Calcium and integrin-binding protein 1; CIB; Calcium- and integrin-binding protein; CIBP; Calmyrin; DNA-PKcs-interacting protein; Kinase-interacting protein; KIP; SNK-interacting protein 2-28; SIP2-28; PRKDCIP; CIB1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 191

• Applicable Applications for CIB1 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Monomer. Interacts with MYO1C. Interacts (via C-terminal region) with PPP3R1 and CACNA1C; the interactions increase upon cardiomyocytes hypertrophy (By similarity). Interacts with the heterodimeric integrin alpha-IIb/beta3 (ITGA2B-ITGB3). Interacts with ITGA2B (via cytoplasmic domain); the interaction is direct and calcium-dependent. Interacts with the protein kinases PLK2/SNK and PRKDC (via the region immediately upstream of the kinase domain). Interacts with PLK3; the interaction inhibits PLK3 kinase activity. Interacts with PSEN2. Interacts (via C-terminus) with F8. Interacts with NBR1 (via C-terminus). Interacts with FEZ1 (via C-terminus). Interacts with UBR5 (via C-terminus); the interaction is sensitive to DNA damage, and may target CIB1 for ubiquitin-mediated degradation. Interacts with IFI6. Interacts with BCL2. Interacts with ITPR3; the interaction occurs in a calcium-dependent manner. Interacts with PTK2/FAK1. Interacts with MAP3K5; the interaction inhibits MAP3K5 activation by phosphorylation, and its subsequent interaction with TRAF2. Isoform 2 interacts with PRKD2 (via N-terminal AP-rich region), PTK2/FAK1 and PAK1. Interacts with TAS1R2 (via C-terminus); the interaction is independent of the myristoylation state of CIB1. Interacts (via C-terminal region) with STMN2 (via the N-terminal region); the interaction is direct, occurs in a calcium-dependent manner and attenuates the STMN2-induced neurite outgrowth inhibition. Interacts with SPHK1, the interaction occurs in a calcium-dependent manner. Interacts with ITGA2B (via C-terminal cytoplasmic tail); the interaction occurs upon platelet aggregation and is stabilized/increased in a calcium and magnesium-dependent manner. Interacts with PAK1 (via N-terminal region); the interaction is direct and occurs in a calcium-dependent manner. Interacts with RAC3 (via C-terminal region); the interaction induces their association with the cytoskeleton upon alpha-IIb/beta3 integrin-mediated adhesion. Interacts with ITGA5 and ITGAV.
• Entry Function: Isoform 2: Plays a regulatory role in angiogenesis and tumor growth by mediating PKD/PRKD2-induced vascular endothelial growth factor A (VEGFA) secretion.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 134047806
• UniProt Accession #: Q99828
• NCBI Official Full Name: Calcium and integrin-binding protein 1
• UniProt Protein Name: Calcium and integrin-binding protein 1
• Protein Family: Calcium and integrin-binding protein
• UniProt Gene Name: CIB1
• UniProt Synonym Gene Names: CIB; KIP; PRKDCIP; CIB; CIBP; KIP; SIP2-28
• UniProt Entry Name: CIB1_HUMAN

Uniprot Description

CIB1: May convert the inactive conformation of integrin alpha- IIb/beta3 to an active form through binding to the integrin cytoplasmic domain. Induces cell migration and spreading mediated through integrin (possibly via focal adhesion complexes). Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. May play a role in regulation of apoptosis. Interacts with and up-regulates PTK2/FAK1 activity. Down regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Participates in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation.

Protein type: Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 15q25.3-q26

Cellular Component: Golgi apparatus; filopodium tip; centrosome; neuron projection; endoplasmic reticulum; nucleoplasm; growth cone; membrane; cell soma; perinuclear region of cytoplasm; lamellipodium; cytoplasm; apical plasma membrane; plasma membrane; nucleus; sarcolemma; vesicle

Molecular Function: protein binding; Ras GTPase binding; protein anchor; calcium ion binding

Biological Process: negative regulation of protein kinase B signaling cascade; apoptosis; negative regulation of microtubule depolymerization; positive regulation of cell adhesion mediated by integrin; positive regulation of metalloenzyme activity; endomitotic cell cycle; positive regulation of cell growth; negative regulation of megakaryocyte differentiation; cytoplasmic microtubule organization and biogenesis; activation of NF-kappaB transcription factor; regulation of cell proliferation; negative regulation of cell proliferation; negative regulation of protein amino acid phosphorylation; cell division; double-strand break repair; positive regulation of cell proliferation; angiogenesis; positive regulation of protein amino acid phosphorylation; platelet formation; positive regulation of cell-matrix adhesion; cell adhesion; response to DNA damage stimulus; regulation of cell division; spermatid development; positive regulation of cell migration; negative regulation of apoptosis