PDHA2 / Pyruvate Dehydrogenase E1 Component Subunit Alpha, Testis-Specific Form Recombinant Protein | PDHA2 recombinant protein

Human PDHA2 / Pyruvate Dehydrogenase E1 Component Subunit Alpha, Testis-Specific Form, Mitochondrial Recombinant Protein

• Gene Names: PDHA2; PDHAL
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: PDHA2 / Pyruvate Dehydrogenase E1 Component Subunit Alpha; Testis-Specific Form; Human PDHA2 / Pyruvate Dehydrogenase E1 Component Subunit Alpha; Mitochondrial Recombinant Protein; Pyruvate dehydrogenase E1 component subunit alpha; testis-specific form; mitochondrial; PDHE1-A type II; PDHAL; PDHA2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 388

• Applicable Applications for PDHA2 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Heterotetramer of two PDHA2 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules.
• Entry Function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 4885543
• NCBI GeneID: 5161
• NCBI Accession #: NP_005381.1
• NCBI GenBank Nucleotide #: NP_005381.1
• UniProt Accession #: P29803
• NCBI Official Full Name: pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
• NCBI Official Synonym Full Names: pyruvate dehydrogenase E1 alpha 2 subunit
• NCBI Official Symbol: PDHA2
• NCBI Official Synonym Symbols: PDHAL
• NCBI Protein Information: pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
• UniProt Protein Name: Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
• Protein Family: Pyruvate dehydrogenase E1 component
• UniProt Gene Name: PDHA2
• UniProt Synonym Gene Names: PDHAL
• UniProt Entry Name: ODPAT_HUMAN

Uniprot Description

PDHA2: a testis-specific mitochondrial matrix enzyme that catalyzes the oxidative decarboxylation of pyruvate, producing acetyl-CoA and CO2. A key enzyme in controlling the balance between lipid and glucose oxidation depending on substrate availability. The pyruvate dehydrogenase (PDH) holoenzyme is a multi-enzyme complex (PDHC) that contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. The activity of PDH is tightly regulated by phosphorylation. The phosphorylation of at least one of three specific serine residues in E1 subunit by PDHK inactivates the PDHC, while dephosphorylation by PDP restores its activity. Expressed in postmeiotic spermatogenic cells.

Protein type: Carbohydrate Metabolism - pyruvate; Oxidoreductase; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.4.1; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis

Chromosomal Location of Human Ortholog: 4q22-q23

Cellular Component: mitochondrial matrix; nucleus; pyruvate dehydrogenase complex

Molecular Function: pyruvate dehydrogenase (acetyl-transferring) activity

Biological Process: acetyl-CoA biosynthetic process from pyruvate; glucose metabolic process; pyruvate metabolic process; tricarboxylic acid cycle