Human TRAF4/TNF Receptor-Associated Factor 4 ELISA Kit | TRAF4 elisa kit

Human TRAF4/TNF Receptor-Associated Factor 4 ELISA Kit

Cat. Num. AAA2890599

• Gene Names: TRAF4; CART1; MLN62; RNF83
• Reactivity: Human
• Synonyms: TRAF4/TNF Receptor-Associated Factor 4; Human TRAF4/TNF Receptor-Associated Factor 4 ELISA Kit; TNF receptor-associated factor 4; Cysteine-rich domain associated with RING and Traf domains protein 1; Metastatic lymph node gene 62 protein; MLN 62; RING finger protein 83; CART1; MLN62; RNF83; TRAF4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human TNF receptor-associated factor 4
• Sequence Length: 470

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRAF4 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22027622
• NCBI GeneID: 9618
• NCBI Accession #: NP_004286.2
• NCBI GenBank Nucleotide #: NM_004295.4
• UniProt Accession #: Q9BUZ4
• NCBI Official Full Name: TNF receptor-associated factor 4
• NCBI Official Synonym Full Names: TNF receptor associated factor 4
• NCBI Official Symbol: TRAF4
• NCBI Official Synonym Symbols: CART1; MLN62; RNF83
• NCBI Protein Information: TNF receptor-associated factor 4
• UniProt Protein Name: TNF receptor-associated factor 4
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF4
• UniProt Synonym Gene Names: CART1; MLN62; RNF83; MLN 62
• UniProt Entry Name: TRAF4_HUMAN

NCBI Description

This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAF4: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract. Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. Belongs to the TNF receptor-associated factor family. B subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q11-q12

Cellular Component: tight junction; cytoskeleton; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; thioesterase binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; WW domain binding; tumor necrosis factor receptor binding; protein kinase binding

Biological Process: regulation of apoptosis; apoptosis; positive regulation of protein kinase activity; respiratory tube development; activation of NF-kappaB-inducing kinase; positive regulation of JNK cascade; protein ubiquitination; respiratory gaseous exchange; signal transduction