Human Interferon regulatory factor 8 ELISA Kit | IRF8 elisa kit

Human Interferon regulatory factor 8 ELISA Kit

Cat. Num. AAA2886345

• Gene Names: IRF8; ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
• Reactivity: Human
• Synonyms: Interferon regulatory factor 8; Human Interferon regulatory factor 8 ELISA Kit; IRF-8; Interferon consensus sequence-binding protein; H-ICSBP; ICSBP; IRF8; ICSBP1; IRF8 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural humanIRF-8. No significant cross-reactivity or interference was observed.
• Sequence Length: 426

• Assay Type: Sandwich
• Detection Range: 31.2-2000 pg/mL
• Sensitivity: 15.61 pg/mL
• Intra-Assay CV: <=6.8%
• Inter-Assay CV: <=8.4%
• Recovery: 106%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRF8 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofhumanIRF-8concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IRF-8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forIRF-8 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IRF-8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IRF-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504567
• NCBI GeneID: 3394
• NCBI Accession #: NP_002154.1
• NCBI GenBank Nucleotide #: NM_002163.2
• UniProt Accession #: Q02556; A0AV82
• Molecular Weight: 48,356 Da
• NCBI Official Full Name: interferon regulatory factor 8
• NCBI Official Synonym Full Names: interferon regulatory factor 8
• NCBI Official Symbol: IRF8
• NCBI Official Synonym Symbols: ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
• NCBI Protein Information: interferon regulatory factor 8
• UniProt Protein Name: Interferon regulatory factor 8
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF8
• UniProt Synonym Gene Names: ICSBP1; IRF-8; H-ICSBP; ICSBP
• UniProt Entry Name: IRF8_HUMAN

NCBI Description

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq, Jul 2008]

Uniprot Description

IRF8: Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with COPS2. By IFNG/IFN-gamma. Predominantly expressed in lymphoid tissues. Belongs to the IRF family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: cytosol; nucleoplasm

Molecular Function: protein binding; RNA polymerase II transcription factor activity, enhancer binding

Biological Process: cytokine and chemokine mediated signaling pathway; defense response to bacterium; defense response to protozoan; immune response; myeloid cell differentiation; negative regulation of transcription from RNA polymerase II promoter; phagocytosis; positive regulation of interferon-gamma production; positive regulation of interleukin-12 production; transcription, DNA-dependent

Disease: Immunodeficiency 32a; Monocyte And Dendritic Cell Deficiency, Autosomal Recessive