Typical Testing Data/Standard Curve (for reference only)
Peroxisome proliferator-activated receptor alpha ELISA Kit | Ppara elisa kit
Rat Peroxisome proliferator-activated receptor alpha ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
nuclear steroid receptor; heterodimerizes with retinoid-X receptor (RXR), and binds to peroxisome proliferator response elements (PPREs) to initiate the transcriptional regulation of target genes [RGD, Feb 2006]
Uniprot Description
PPAR-alpha: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Belongs to the nuclear hormone receptor family. NR1 subfamily.
Protein type: DNA-binding; Nuclear receptor
Cellular Component: nucleus
Molecular Function: DNA binding; drug binding; ligand-dependent nuclear receptor activity; lipid binding; NFAT protein binding; phosphatase binding; protein binding; protein complex binding; protein domain specific binding; receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; ubiquitin conjugating enzyme binding; zinc ion binding
Biological Process: behavioral response to nicotine; circadian regulation of gene expression; epidermis development; fatty acid metabolic process; heart development; intracellular receptor-mediated signaling pathway; lipoprotein metabolic process; negative regulation of appetite; negative regulation of blood pressure; negative regulation of inflammatory response; negative regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; positive regulation of fatty acid oxidation; positive regulation of gluconeogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of circadian rhythm; regulation of fatty acid metabolic process; regulation of gene expression; regulation of transcription, DNA-dependent; response to hypoxia; response to insulin stimulus; steroid hormone mediated signaling; transcription from RNA polymerase II promoter; wound healing
Research Articles on Ppara
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Product Notes
The Rat Ppara ppara (Catalog #AAA2884186) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2884186 ELISA Kit recognizes Rat Ppara. It is sometimes possible for the material contained within the vial of "Peroxisome proliferator-activated receptor alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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