Mouse Mitogen-activated protein kinase kinase kinase 5 ELISA Kit | Map3k5 elisa kit

Mouse Mitogen-activated protein kinase kinase kinase 5 ELISA Kit

Cat. Num. AAA2883858

• Gene Names: Map3k5; ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik
• Reactivity: Mouse
• Synonyms: Mitogen-activated protein kinase kinase kinase 5; Mouse Mitogen-activated protein kinase kinase kinase 5 ELISA Kit; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Map3k5; Ask1; Mekk5; 2.7.11.25; Map3k5 elisa kit

• Reactivity: Mouse
• Sequence Length: 1380

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/mL
• Sensitivity: 0.156 ng/mL
• Intra-Assay CV: <=7.0%
• Inter-Assay CV: <=9.0%
• Recovery: 89%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 171846249
• NCBI GeneID: 26408
• NCBI Accession #: NP_032606.4
• NCBI GenBank Nucleotide #: NM_008580.4
• UniProt Accession #: O35099; Q14AY5
• Molecular Weight: 154,512 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 5
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 5
• NCBI Official Symbol: Map3k5
• NCBI Official Synonym Symbols: ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 5
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 5
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: Map3k5
• UniProt Synonym Gene Names: Ask1; Mekk5; ASK-1; MEK kinase 5; MEKK 5
• UniProt Entry Name: M3K5_MOUSE

Uniprot Description

ASK1: a MAP kinase kinase kinase of the STE11 family, apoptosis signal-regulating kinase 1 (ASK1) plays essential roles in stress-induced apoptosis. Activated by various stressors, including oxidative stress, endoplasmic reticulum stress, and calcium overload, as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) and lipopolysaccharide (LPS). In turn, ASK1 phosphorylates and activates two different subgroups of MAP kinase kinases, MKK4/SEK1 and MKK3/MKK6, which in turn activate stress-activated protein kinases (JNKs) and p38 subgroups of MAP kinases, respectively. Overexpression activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation as well as cell death. Oxidative stress induces the activation of ASK1, which correlate with ASK1 activity and ASK1-dependent apoptosis. Required for the innate immune response, which is essential for host defense against a wide range of pathogens.

Protein type: Protein kinase, STE; Kinase, protein; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; external side of plasma membrane

Molecular Function: ATP binding; kinase activity; magnesium ion binding; MAP kinase kinase kinase activity; metal ion binding; nucleotide binding; protein binding; protein homodimerization activity; protein kinase activity; protein kinase binding; protein phosphatase binding; protein serine/threonine kinase activity; transferase activity

Biological Process: activation of MAPK activity; activation of MAPKK activity; apoptosis; immune system process; induction of apoptosis by oxidative stress; innate immune response; JNK cascade; MAPKKK cascade; phosphorylation; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of myoblast differentiation; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation