Mouse Muscarinic acetylcholine receptor M3 ELISA Kit | Chrm3 elisa kit

Mouse Muscarinic acetylcholine receptor M3 ELISA Kit

Cat. Num. AAA2883768

• Gene Names: Chrm3; M3; M3R; Chrm-3
• Reactivity: Mouse
• Synonyms: Muscarinic acetylcholine receptor M3; Mouse Muscarinic acetylcholine receptor M3 ELISA Kit; Mm3 mAChR; Chrm3; Chrm-3; Chrm3 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse Muscarinic acetylcholine receptor M3
• Sequence Length: 589

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: < 0.065 ng/mL
• Intra-assay Precision: <=4.4%
• Inter-assay Precision: <=8.2%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Chrm3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15149489
• NCBI GeneID: 12671
• NCBI Accession #: NP_150372.1
• NCBI GenBank Nucleotide #: NM_033269.4
• UniProt Accession #: Q9ERZ3; Q64055
• Molecular Weight: 66,212 Da
• NCBI Official Full Name: muscarinic acetylcholine receptor M3
• NCBI Official Synonym Full Names: cholinergic receptor, muscarinic 3, cardiac
• NCBI Official Symbol: Chrm3
• NCBI Official Synonym Symbols: M3; M3R; Chrm-3
• NCBI Protein Information: muscarinic acetylcholine receptor M3
• UniProt Protein Name: Muscarinic acetylcholine receptor M3
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: Chrm3
• UniProt Synonym Gene Names: Chrm-3
• UniProt Entry Name: ACM3_MOUSE

Uniprot Description

mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Cellular Component: asymmetric synapse; cell junction; dendrite; integral to membrane; integral to plasma membrane; membrane; nerve terminal; plasma membrane; postsynaptic membrane; synapse

Molecular Function: acetylcholine binding; acetylcholine receptor activity; drug binding; G-protein coupled acetylcholine receptor activity; G-protein coupled receptor activity; signal transducer activity

Biological Process: acetylcholine receptor signaling, muscarinic pathway; digestion; G-protein coupled receptor protein signaling pathway; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; muscarinic acetylcholine receptor, phospholipase C activating pathway; positive regulation of smooth muscle contraction; regulation of vascular smooth muscle contraction; regulation of vasoconstriction; saliva secretion; signal transduction; smooth muscle contraction; synaptic transmission, cholinergic