IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human, mouse SRPK2 Polyclonal Antibody | anti-SRPK2 antibody
SRPK2 Antibody (C-term)
Gene Names
SRPK2; SFRSK2
Reactivity
Human, mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SRPK2, Antibody; SRPK2 Antibody (C-term); SRSF protein kinase 2; SFRS protein kinase 2; Serine/arginine-rich protein-specific kinase 2; SR-protein-specific kinase 2; SRSF protein kinase 2 N-terminal; SRSF protein kinase 2 C-terminal; SRPK2 {ECO:0000312|EMBL:AAH685471}; anti-SRPK2 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SRPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465-495 amino acids from the C-terminal region of human SRPK2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
465-495
Sequence Length
688
Applicable Applications for anti-SRPK2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Serine/arginine-rich protein-specific kinasewhich specifically phosphorylates itssubstrates at serine residues located inregions rich in arginine/serine dipeptides,known as RS domains and is involved in thephosphorylation of SR splicing factors and theregulation of splicing. Promotes neuronalapoptosis by up-regulating cyclin-D1 (CCND1)expression. This is done by thephosphorylation of SRSF2, leading to thesuppression of p53/TP53 phosphorylationthereby relieving the repressive effect ofp53/TP53 on cyclin-D1 (CCND1) expression.Phosphorylates ACIN1, and redistributes itfrom the nuclear speckles to thenucleoplasm, resulting in cyclin A1 but notcyclin A2 up- regulation. Plays an essentialrole in spliceosomal B complex formation viathe phosphorylation of DDX23/PRP28. Canmediate hepatitis B virus (HBV) core proteinphosphorylation. Plays a negative role in theregulation of HBV replication through amechanism not involving the phosphorylationof the core protein but by reducing thepackaging efficiency of the pregenomic RNA(pgRNA) without affecting the formation ofthe viral core particles.
Cellular Location
Cytoplasm. Nucleus. Note=Shuttles betweenthe nucleus and the cytoplasm. KAT5/TIP60inhibits its nuclear translocation.Phosphorylation at Thr-492 by PKB/AKT1promotes nuclear translocation.
Tissue Location
Highly expressed in brain, moderatelyexpressed in heart and skeletal muscle and atlow levels in lung, liver, and kidney generally the g phosphate of ATP, onto anacceptor amino acid in a substrate protein. Bythis basic mechanism, protein kinases mediatemost of the signal transduction in eukaryoticcells, regulating cellular metabolism,transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis,and differentiation. With more than 500 gene products, the protein kinase family is one of thelargest families of proteins in eukaryotes. Thefamily has been classified in 8 major groups based on sequence comparison of their tyrosine(PTK) or serine/threonine (STK) kinase catalyticdomains. The STE group (homologs of yeastSterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase(MAPK) cascade families (Ste7/MAP2K,Ste11/MAP3K, and Ste20/MAP4K). MAP kinasecascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of anti-SRPK2 Pab (AAA288175) in mouse thymus tissue lysate. SRPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
WB (Western Blot)
(Western blot analysis of anti-SRPK2 Pab (AAA288175) in mouse thymus tissue lysate. SRPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Related Product Information for anti-SRPK2 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Product Categories/Family for anti-SRPK2 antibody
References
Blume-Jensen P, et al. Nature 2001. 411: 355.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77527
NCBI Official Full Name
SRSF protein kinase 2 isoform b
NCBI Official Synonym Full Names
SRSF protein kinase 2
NCBI Official Symbol
SRPK2
NCBI Official Synonym Symbols
SFRSK2
NCBI Protein Information
SRSF protein kinase 2
UniProt Protein Name
SRSF protein kinase 2
UniProt Gene Name
SRPK2
UniProt Synonym Gene Names
SR-protein-specific kinase 2
UniProt Entry Name
SRPK2_HUMAN
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Product Notes
The SRPK2 srpk2 (Catalog #AAA288175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 465-495. The SRPK2 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SRPK2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SRPK2 srpk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRPK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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