Sandwich ELISA
(Plate was coated with Glargine Insulin at 1.25 ug/ml in PBS, and then incubated with Anti-Glargine Insulin polyclonal antibody from 0.001 ug/ml to 0.5 ug/ml. The secondary antibody, HRP conjugated goat anti-Guinea pig IgG, were used at 1:6000 dilution.)
Guinea Pig Glargine Insulin Polyclonal Antibody
Anti-Glargine Insulin Polyclonal Antibody
Applications
ELISA
Purity
This antibody is purified through a antigen affinity purification column, followed by dialysis against PBS.
Synonyms
Glargine Insulin; Polyclonal Antibody; Anti-Glargine Insulin Polyclonal Antibody; Anti-Glargine Insulin polyclonal antibody; anti-Glargine Insulin antibody
Host
Guinea Pig
Clonality
Polyclonal
Specificity
Guinea pig polyclonal antibody raised against Glargine Insulin.
Purity/Purification
This antibody is purified through a antigen affinity purification column, followed by dialysis against PBS.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Applicable Applications for anti-Glargine Insulin antibody
ELISA (EIA)
Application Notes
Sandwich ELISA
Antigen Source
Insulin glargine, marketed under the names Lantus, among others, is a long-acting basal insulin analogue, given once daily to help control the blood sugar level of those with diabetes. Insulin glargine has a substitution of glycine for asparagine at N21 (Asn21) and two arginines added to the carboxy terminal of B chain. The arginine amino acids shift the isoelectric point from a pH of 5.4 to 6.7, making the molecule more soluble at an acidic pH and less soluble at physiological pH. The isoelectric shift also allows for the subcutaneous injection of a clear solution. The glycine substitution prevents deamidation of the acid-sensitive asparagine at acidic pH. In the neutral subcutaneous space, higher-order aggregates form, resulting in a slow, peakless dissolution and absorption of insulin from the site of injection. It can achieve a peakless level for at least 24 hours. Molecular formula: C267H404N72O78S6 Molecular weight: 6063
Preparation and Storage
Maintain refrigerated at 2-8 degrees C for up to 2 weeks. For long term storage store at -20 degrees C in small aliquots to prevent freeze-thaw cycles.
Sandwich ELISA
(Plate was coated with Glargine Insulin at 1.25 ug/ml in PBS, and then incubated with Anti-Glargine Insulin polyclonal antibody from 0.001 ug/ml to 0.5 ug/ml. The secondary antibody, HRP conjugated goat anti-Guinea pig IgG, were used at 1:6000 dilution.)
Sandwich ELISA
(Plate was coated with Glargine Insulin at 1.25 ug/ml in PBS, and then incubated with Anti-Glargine Insulin polyclonal antibody from 0.001 ug/ml to 0.5 ug/ml. The secondary antibody, HRP conjugated goat anti-Guinea pig IgG, were used at 1:6000 dilution.)
Related Product Information for anti-Glargine Insulin antibody
Purified Guinea pig polyclonal Antibody (Pab)
Similar Products
Product Notes
The Glargine Insulin (Catalog #AAA28808) is an Antibody produced from Guinea Pig and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glargine Insulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Sandwich ELISA. Researchers should empirically determine the suitability of the Glargine Insulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glargine Insulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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