Mouse Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog ELISA Kit | Litaf elisa kit

Mouse Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog ELISA Kit

Cat. Num. AAA288002

• Gene Names: Litaf; TBX1; C85531; N4WBP3; 3222402J11Rik
• Reactivity: Mouse
• Synonyms: Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog; Mouse Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog ELISA Kit; Litaf elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse LPS-induced TNF-alpha factor homolog. No significant cross-reactivity or interference was observed.
• Sequence Length: 148

• Assay Type: Sandwich
• Detection Range: 15.6-1000 pg/ml
• Sensitivity: 7.88 pg/mL
• Intra-Assay CV: <=6.1%
• Inter-Assay CV: <=8.5%
• Recovery: 95%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Litaf elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LPS-induced TNF-alpha factor homolog. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LPS-induced TNF-alpha factor homolog and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LPS-induced TNF-alpha factor homolog, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LPS-induced TNF-alpha factor homolog in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9910578
• NCBI GeneID: 56722
• NCBI Accession #: NP_064364.1
• NCBI GenBank Nucleotide #: NM_019980.2
• UniProt Accession #: Q9JLJ0; Q9EQI0
• Molecular Weight: 16,946 Da
• NCBI Official Full Name: lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
• NCBI Official Synonym Full Names: LPS-induced TN factor
• NCBI Official Symbol: Litaf
• NCBI Official Synonym Symbols: TBX1; C85531; N4WBP3; 3222402J11Rik
• NCBI Protein Information: lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
• UniProt Protein Name: Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
• Protein Family: Lipopolysaccharide-induced tumor necrosis factor-alpha factor
• UniProt Gene Name: Litaf
• UniProt Synonym Gene Names: N4wbp3; Tbx1; LPS-induced TNF-alpha factor homolog; mEET
• UniProt Entry Name: LITAF_MOUSE

Uniprot Description

LITAF: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF- alpha) gene expression. Interacts with NEDD4. Interacts with WWOX. Isoform 2 may interact with STAT6. By bacterial lipopolysaccharides (LPS) or p53/TP53. In monocytes by the Bacillus Calmette-Guerin (BCG). Ubiquitously and abundantly expressed. Expressed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor

Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; lysosomal membrane; lysosome; membrane; nucleoplasm; plasma membrane

Molecular Function: protein binding; signal transducer activity; WW domain binding

Biological Process: apoptosis; negative regulation of NF-kappaB import into nucleus; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of cytokine production; regulation of transcription, DNA-dependent; response to lipopolysaccharide; signal transduction; transcription, DNA-dependent