Human Motile sperm domain-containing protein 3 (MOSPD3) ELISA Kit | MOSPD3 elisa kit

Human Motile sperm domain-containing protein 3 (MOSPD3) ELISA Kit

Cat. Num. AAA287539

• Gene Names: MOSPD3; CDS3; NET30
• Reactivity: Human
• Synonyms: Motile sperm domain-containing protein 3 (MOSPD3); Human Motile sperm domain-containing protein 3 (MOSPD3) ELISA Kit; CDS3; NET30; MOSPD3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MOSPD3. No significant cross-reactivity or interference between Human MOSPD3 and analogues was observed.
• Sequence Length: 235

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MOSPD3 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate MOSPD3 in samples. An antibody specific for MOSPD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MOSPD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MOSPD3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOSPD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Pall et al. (2004) cloned mouse Mospd3 and identified the human MOSPD3 gene. The deduced 235-amino acid MOSPD3 protein contains an N-terminal major sperm protein domain and 2 transmembrane domains. By genomic sequence analysis, Pall et al. (2004) mapped the MOSPD3 gene to chromosome 7q22. They mapped the mouse Mospd3 gene to a region of chromosome 5 that shows homology of synteny to human chromosome 7q22. Pall et al. (2004) found that homozygous Mospd3-null mice had a right ventricle defect with 50% probability of neonatal lethality. The phenotypic defect was dependent on the genetic background, indicating the presence of modifier loci

NCBI and Uniprot Product Information

• NCBI GI #: 93141012
• NCBI GeneID: 64598
• NCBI Accession #: NP_001035186.1
• NCBI GenBank Nucleotide #: NM_001040097.1
• UniProt Accession #: O75425; O75423; O75424; A4D2D1; A6NG17; C9JE89; D6W5W1
• Molecular Weight: 24,448 Da
• NCBI Official Full Name: motile sperm domain-containing protein 3 isoform a
• NCBI Official Synonym Full Names: motile sperm domain containing 3
• NCBI Official Symbol: MOSPD3
• NCBI Official Synonym Symbols: CDS3; NET30
• NCBI Protein Information: motile sperm domain-containing protein 3
• UniProt Protein Name: Motile sperm domain-containing protein 3
• Protein Family: Motile sperm domain-containing protein
• UniProt Gene Name: MOSPD3

NCBI Description

This gene encodes a multi-pass membrane protein with a major sperm protein (MSP) domain. The deletion of a similar mouse gene is associated with defective cardiac development and neonatal lethality. Alternate transcriptional splice variants, encoding different isoforms, have been described. [provided by RefSeq, Jul 2008]