Rat NTF2-related export protein 2 (NXT2) ELISA Kit | NXT2 elisa kit
Rat NTF2-related export protein 2 (NXT2) ELISA Kit
Gene Names
Nxt2; RGD1560204
Reactivity
Rat
Synonyms
NTF2-related export protein 2 (NXT2); Rat NTF2-related export protein 2 (NXT2) ELISA Kit; RP4-596C15.2; P15-2; NXT2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat NXT2. No significant cross-reactivity or interference between Rat NXT2 and analogues was observed.
Sequence Length
142
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other Biological Fluids
Detection Wavelength
450 nm
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%)
= SD/meanX100
Intra-Assay
CV
Inter-Assay
CV
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for NXT2 elisa kit
Description: This assay employs a two-site sandwich ELISA to quantitate NXT2 in samples. An antibody specific for NXT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NXT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NXT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NXT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Export of mRNAs from the nucleus involves a number of proteins, including nucleoporins, RNA-binding proteins, an RNA helicase (DDX19), and nuclear pore complex-associated proteins, such as NXF1 and RAE1. By searching EST databases for homologs of p15 (NXT1), Herold et al. (2000) identified cDNAs encoding 2 variants of NXT2, the products of which they termed p15-2a and p15-2b. The variants differ in their 5-prime exons. The deduced 15-2a and 15-2b proteins contain 142 and 114 amino acids, respectively, and are highly homologous to Drosophila and worm proteins, as well as to NXT1. Fluorescence microscopy demonstrated NXT2 expression predominantly in the nucleoplasm and nuclear envelope, with a fraction found in the cytoplasm. Like NXT1, NXT2 was excluded from the nucleolus.
Overview: Export of mRNAs from the nucleus involves a number of proteins, including nucleoporins, RNA-binding proteins, an RNA helicase (DDX19), and nuclear pore complex-associated proteins, such as NXF1 and RAE1. By searching EST databases for homologs of p15 (NXT1), Herold et al. (2000) identified cDNAs encoding 2 variants of NXT2, the products of which they termed p15-2a and p15-2b. The variants differ in their 5-prime exons. The deduced 15-2a and 15-2b proteins contain 142 and 114 amino acids, respectively, and are highly homologous to Drosophila and worm proteins, as well as to NXT1. Fluorescence microscopy demonstrated NXT2 expression predominantly in the nucleoplasm and nuclear envelope, with a fraction found in the cytoplasm. Like NXT1, NXT2 was excluded from the nucleolus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,366 Da
NCBI Official Full Name
NTF2-related export protein 2
NCBI Official Synonym Full Names
nuclear transport factor 2-like export factor 2
NCBI Official Symbol
Nxt2
NCBI Official Synonym Symbols
RGD1560204
NCBI Protein Information
NTF2-related export protein 2
UniProt Protein Name
NTF2-related export protein 2
Protein Family
UniProt Gene Name
Nxt2
Uniprot Description
Regulator of protein export for NES-containing proteins. Also plays a role in mRNA nuclear export ().
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Product Notes
The Rat NXT2 nxt2 (Catalog #AAA287512) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA287512 ELISA Kit recognizes Rat NXT2. It is sometimes possible for the material contained within the vial of "NTF2-related export protein 2 (NXT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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