Rat Serine/threonine-protein kinase 12 (AURKB) ELISA Kit | AURKB elisa kit

Rat Serine/threonine-protein kinase 12 (AURKB) ELISA Kit

Cat. Num. AAA287367

• Gene Names: Aurkb; Aim1; ARK-2; STK-1; Stk12
• Reactivity: Rat
• Synonyms: Serine/threonine-protein kinase 12 (AURKB); Rat Serine/threonine-protein kinase 12 (AURKB) ELISA Kit; AIK2; AIM-1; AIM1; ARK2; AurB; IPL1; STK12; STK5; aurkb-sv1; aurkb-sv2; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora-1; aurora-B; serine/threonine kinase 12; AURKB elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat AURKB. No significant cross-reactivity or interference between Rat AURKB and analogues was observed.
• Sequence Length: 343

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for AURKB elisa kit

This assay employs a two-site sandwich ELISA to quantitate AURKB in samples. An antibody specific for AURKB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AURKB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AURKB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AURKB bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 16758580
• NCBI GeneID: 114592
• NCBI Accession #: NP_446201.1
• NCBI GenBank Nucleotide #: NM_053749.1
• UniProt Accession #: O55099; Q4V8N1
• Molecular Weight: 39,234 Da
• NCBI Official Full Name: aurora kinase B
• NCBI Official Synonym Full Names: aurora kinase B
• NCBI Official Symbol: Aurkb
• NCBI Official Synonym Symbols: Aim1; ARK-2; STK-1; Stk12
• NCBI Protein Information: aurora kinase B
• UniProt Protein Name: Aurora kinase B
• Protein Family: Aurora kinase
• UniProt Gene Name: Aurkb
• UniProt Synonym Gene Names: Aik2; Aim1; Airk2; Ark2; Stk1; Stk12; Stk5; ARK-2; Aurora-related kinase 2

NCBI Description

kinase; involved in proper progression of cytokinesis [RGD, Feb 2006]

Uniprot Description

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, HASPIN and histone H3. A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes ().